Overexpression of profilin causes abnormal somites and notochord formation in zebrafish
Date Issued
2005
Date
2005
Author(s)
Wu, Chieh-Tui
DOI
en-US
Abstract
To study the roles of profilins in embryogenesis, we have identified a profilin II gene in zebrafish. By RT-PCR, a 423 b.p. profilin II transcript was highly expressed during cleavage and blastula periods as maternal transcripts, disappearing and a zygotic expression was not observed until 24 hours post fertilization (hpf). Profilin II expression was observed in all adult tissues examined. By whole-mount in situ hybridization, profilin II was ubiquitously expressed in all blastomeres of early embryos, but localized mainly in brain and neuronal tissues after 24 hpf. Overexpression of profilin II-EGFP caused the partial inhibition of somitogenesis and the distortion of notochord, which resulted in the malformation and truncation of trunk and tail. Furthermore, morpholino knock-down of profilin II induced a shorten body axis and a contorted tail. These results suggest that a normal expression of profilin II is required for the proper development of neuron, somite and possibly body axis formation in zebrafish.
Subjects
胚發育
肌動蛋白
斑馬魚
development
notochord
somite
actin
profilin
zebrafish
Type
other
File(s)![Thumbnail Image]()
Loading...
Name
ntu-94-R91243013-1.pdf
Size
23.31 KB
Format
Adobe PDF
Checksum
(MD5):decf278dcddfcc378b0713babafd6930