https://scholars.lib.ntu.edu.tw/handle/123456789/142408
Title: | 水產動物淡海水適應之生理機制─魚類對鹽度之能量代謝反應及調節機制之研究 | Authors: | 郭欽明 | Keywords: | 點帶石斑;壓迫生理反應;鹽度;Epinephelus coioides;Stress responses;Salinity | Issue Date: | 31-Jul-2001 | Publisher: | 臺北市:國立臺灣大學漁業科學研究所 | Abstract: | 本研究以急劇的鹽度刺激方式來探討不同 鹽度刺激對點帶石斑( Epinephelus coioides) 之生理反應之影響,包括血液、滲透壓調 節及醣類代謝等。馴化於25 ppt之石斑魚, 分別移入不同的鹽度5、15、25、35、45 ppt, 進行刺激試驗,探討其在急劇鹽度變化下 的滲透壓調節情形,包括血容比、血紅素、 滲透壓、鈉、鉀、鈣、鎂、氯離子、及鰓 絲Na+, K+-ATPase的變化。在血液中血容比 及血紅素含量上並無明顯變化。血漿中滲 透壓值於低張環境下(5,15 ppt)其滲透壓值 會出現暫時性低滲透壓現象,然後於6小時 以後則回復初始值,相反的,於高張環境 下(35, 45 ppt),則出現暫時性高滲透壓現 象,於12小時以後,再調降為原來滲透壓 值。低鹽度(5 ppt)刺激下之血糖量上升,僅 出現在刺激早期,至於高鹽刺激下,48小 時內均維持在高血糖(hyperglycemia)現 象。說明了鹽度刺激,對石斑魚表現了明 顯的壓迫反應及能量需求迫切性。血乳酸 值在低鹽(5 ppt)及高鹽(35、45 ppt)的刺激 下,1小時其血漿中乳酸含量明顯上升,再 隨著時間的持續而有下降的趨勢,意謂著 刺激初期以無氧代謝產生能量以應調適所 需。 The physiological responses of the groupers, Epinephelus coioides under acute salinity shock were monitored. The study is focused on hematological responses, osmoregulation and carbohydrate metabolism. The acclimated groupers at the salinity of 25 ppt were directly transferred to varying salinities of 5, 15, 25, 35 and 45 ppt, and the osmoregulation of the groupers under the salinity shock was monitored. The parameters monitored included hematocrit, hemoglobin, plasma osmolarity and ion composition, and Na+-K+ -activated ATPase activity in the gills. Under hypoosomotic environment (5 and 15 ppt), hypoosmorality was characteristic responses in the initial phase of salinity shock, but the plasma osmolarity recovered to the normal level after 6hr. A temporary hyperosmorality was noted under hyperosmotic environments in the initial period up to 12 hr. Furthermore, Na+-K+ ATPase activity was elevated under hyperosmotic conditions, and vice versa. The physiological responses and regulation were demonstrated in these parameters monitored. Hyperglycemia was found to be a common indicator for stress response in the groupers exposed to varying salinities. Plasma glucose was elevated within 48 hr under hight salinity condition, and only in a short initial phase of low salinity conditions. The observation not only implied the occurrence of stress responses, but also increased demands for energy for physiological regulation and compensation. Hyperlactocemia was another indicator of the stress responses, and significant increase in the plasma lactate was detectable within 1 hr after the shock in all cases, followed by gradual declines thereafter. This suggested that the groupers rely heavily on the energy supplies through anaerobic pathway in the initial phase of physiological compensation. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/20546 | Other Identifiers: | 892313B002231 | Rights: | 國立臺灣大學漁業科學研究所 |
Appears in Collections: | 漁業科學研究所 |
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892313B002231.pdf | 128.87 kB | Adobe PDF | View/Open |
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