DC 欄位 | 值 | 語言 |
dc.contributor.author | 蔡懷楨 | zh_TW |
dc.creator | 蔡懷楨 | zh_TW |
dc.date | 2002-07-31 | zh_TW |
dc.date.accessioned | 2006-07-26T02:13:13Z | - |
dc.date.accessioned | 2018-07-06T05:29:31Z | - |
dc.date.available | 2006-07-26T02:13:13Z | - |
dc.date.available | 2018-07-06T05:29:31Z | - |
dc.date.issued | 2002-07-31 | - |
dc.identifier | 902313B002260 | en |
dc.identifier.uri | http://ntur.lib.ntu.edu.tw//handle/246246/20552 | - |
dc.description.abstract | Myf-5, a transcription factor that controls muscle differentiation, is expressed in
somites during early embryogenesis. However, gene regulation of myf-5 is poorly
understood and detailed functional analysis of the regulatory cis-elements is needed.
In zebrafish, the myf-5 upstream sequence from -82 to -62 (-82/-62) was fused with a
basal promoter and transferred to fertilized zebrafish eggs. The -82/-62 cassette
drove GFP reporter gene expression specifically in the somites. Moreover, GFP
signals were detected exclusively in the somites of 28-hpf embryos derived from eggs
injected with 5×(-82/-62)/CMV/EGFP, which contained 5 copies of the -82/-62
cassette inserted within CMV promoter/enhancer. Thus, the -82/-62 cassette has
dual functions: to enhance somite-specific expression and to repress non-specific
expression during the early development of zebrafish embryos. This cassette
sequence was highly conserved in mouse myf-5 and had the same function. Mutated
sequence analysis and electrophoretic mobility shift assay of -82/-62 cassette showed
that the -70/-62 sequence is the major element for controlling myf-5 specificity and for
forming the DNA-protein complex. However, the putative CCAAT-like box, located
at -66/-62, could not direct somite-specific expression. Therefore, the -70/-67
sequence is essential for controlling somite-specific expression and the CCAAT-like
box is essential for activating gene transcription. | en |
dc.format | application/pdf | en |
dc.format.extent | 505788 bytes | en |
dc.format.mimetype | application/pdf | en |
dc.language | zh_TW | zh_TW |
dc.language.iso | zh_TW | zh_TW |
dc.publisher | 臺北市:國立臺灣大學漁業科學研究所 | zh_TW |
dc.rights | 國立臺灣大學漁業科學研究所 | zh_TW |
dc.subject | cis-element | en |
dc.subject | EMSA | en |
dc.subject | GFP | en |
dc.subject | myf-5 | en |
dc.subject | somite-specific expression | en |
dc.subject | transgenic
zebrafish | en |
dc.title | 魚類肌肉調控蛋白MYF-5及MYOGENIN的生物特性:在胚胎早期發育中的角色及互動(2/2) | zh_TW |
dc.type | journal article | en |
dc.identifier.uri.fulltext | http://ntur.lib.ntu.edu.tw/bitstream/246246/20552/1/902313B002260.pdf | - |
dc.coverage | 計畫年度:90;起迄日期:2001-08-01/2002-07-31 | zh_TW |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.languageiso639-1 | zh_TW | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.fulltext | with fulltext | - |
顯示於: | 漁業科學研究所
|