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  4. 微囊藻毒與蛋白質磷酸酵素間之共價鍵形成的動力學分析
 
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微囊藻毒與蛋白質磷酸酵素間之共價鍵形成的動力學分析

Date Issued
2004-07-31
Date
2004-07-31
Author(s)
周宏農  
DOI
922311B002103
URI
http://ntur.lib.ntu.edu.tw//handle/246246/20561
Abstract
Formation of microcystin-phosphatase adducts formation is a clear indication of microcystin attach on phosphatases. However, the kinetics of microcystin-phosphatase adduct formation has been controversial due to the uses of different microcystin isoforms and measuring methodologies. Here, we used matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to measure the changes in relative intensities of molecular weight signals upon mixing of phosphatase and microcystin, which allowed us to monitor the kinetics of covalent adduct formation. Our data clearly indicated that the shifts of signals upon adduct formation and a slow and steady covalent interaction between MCYST-LR and PP-1c were observed. These observations are in agreement with the results of Craig et al (1996) and support their “two-step mechanism” proposed to elucidate the interaction between PP-1c and microcystins.
Publisher
臺北市:國立臺灣大學漁業科學研究所
Type
journal article
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