https://scholars.lib.ntu.edu.tw/handle/123456789/142589
標題: | miR-122 targets an anti-apoptotic gene, Bcl-w, in human hepatocellular carcinoma cell lines | 作者: | Lin, Cliff Ji-Fan Gong, Hong-Yi Tseng, Hung-Chia Wang, Wei-Lun Wu, Jen-Leih |
關鍵字: | Bcl-w; Hepatocellular carcinoma; miR-122 | 公開日期: | 2008 | 起(迄)頁: | 315-320 | 來源出版物: | Biochemical and Biophysical Research Communications | 摘要: | miR-122, a hepato-specific microRNA (miRNA), is frequently down-regulated in human hepatocellular carcinoma (HCC). In an effort to identify novel miR-122 targets, we performed an in silico analysis and detected a putative binding site in the 3′-untranslated region (3′-UTR) of Bcl-w, an anti-apoptotic Bcl-2 family member. In the HCC-derived cell lines, Hep3B and HepG2, we confirmed that miR-122 modulates Bcl-w expression by directly targeting binding site within the 3′-UTR. The cellular mRNA and protein levels of Bcl-w were repressed by elevated levels of miR-122, which subsequently led to reduction of cell viability and activation of caspase-3. Thus, Bcl-w is a direct target of miR-122 that functions as an endogenous apoptosis regulator in these HCC-derived cell lines. ? 2008 Elsevier Inc. All rights reserved. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/162858 | DOI: | 10.1016/j.bbrc.2008.07.154 | SDG/關鍵字: | caspase 3; microRNA; protein bcl w; 3' untranslated region; apoptosis; article; Bcl w gene; binding site; cancer cell culture; cell viability; enzyme activation; gene; gene targeting; human; human cell; liver cell carcinoma; mir 122 gene; nucleotide sequence; priority journal; protein blood level; 3' Untranslated Regions; Apoptosis; Apoptosis Regulatory Proteins; Base Sequence; Carcinoma, Hepatocellular; Caspase 3; Cell Survival; Down-Regulation; Enzyme Activation; Genetic Vectors; Humans; Liver Neoplasms; MicroRNAs; Molecular Sequence Data; RNA, Small Interfering; Transfection |
顯示於: | 漁業科學研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。