Repository logo
  • English
  • 中文
Log In
Have you forgotten your password?
  1. Home
  2. College of Life Science / 生命科學院
  3. Biochemical Sciences / 生化科學研究所
  4. Identification of 5′-upstream region of pufferfish ribosomal protein L29 gene as a strong constitutive promoter to drive GFP expression in zebrafish
 
  • Details

Identification of 5′-upstream region of pufferfish ribosomal protein L29 gene as a strong constitutive promoter to drive GFP expression in zebrafish

Journal
Biochemical and Biophysical Research Communications
Journal Volume
314
Journal Issue
1
Pages
249-258
Date Issued
2004
Date
2004
Author(s)
Chang, Ming-Huang
Chou, Chih-Ming
Hsieh, Yueh-Chun
Lu, I-Ching
M.Kothai Nachiar Devi
Chang, Juei-Pei
Kuo, Tzong-Fu  
Huang, Chang-Jen
DOI
10.1016/j.bbrc.2003.12.080
URI
http://ntur.lib.ntu.edu.tw//handle/246246/162976
http://ntur.lib.ntu.edu.tw/bitstream/246246/162976/1/19.pdf
Abstract
The genomic structure of Tetraodon fluviatilis L29 gene was determined and its promoter activity was analyzed in COS-1 cells and zebrafish embryos. The TfL29 gene comprises four exons and three introns, spanning approximately 1.7kb. The 5′-upstream 2.2-kb of the first exon contains 10 E-boxes and many putative binding motifs for transcription factors GATA-1, AML-1a, c-Myb, Oct-1, CdxA, and NRF-2. Promoter activity assay showed that the distal 2.2-kb fragment not only had high luciferase activity in COS-1 cells, but also strong and ubiquitous GFP expression in a variety of tissues in zebrafish embryos. On the other hand, there are no TATA or CAAT boxes within a 300-bp region upstream from the transcription initiation site. Although this region has high luciferase activity in COS-1 cells, it is not sufficient to drive GFP expression in zebrafish embryos. In this proximal 300-bp region, there are two E-boxes, two CdxA sites, and one NRF-2 site that is immediately downstream of the transcription start site. ? 2003 Elsevier Inc. All rights reserved.
Other Subjects
protein c Myb; ribosome protein; TATA binding protein; transcription factor; transcription factor GATA 1; transcription factor RUNX1; green fluorescent protein; hybrid protein; photoprotein; Rpl29 protein, mouse; animal cell; article; cell strain COS1; controlled study; enzyme activity; exon; fish genetics; gene expression regulation; gene identification; gene location; gene structure; genome analysis; intron; nonhuman; nucleotide sequence; priority journal; promoter region; protein expression; protein motif; puffer fish; transcription initiation site; zebra fish; amino acid sequence; animal; Caenorhabditis elegans; chemistry; comparative study; Drosophila; genetics; human; metabolism; molecular genetics; sequence alignment; sequence analysis; sequence homology; species difference; structure activity relation; Tetraodontiformes; transactivation; Danio rerio; Tetraodon; Tetraodon fluviatilis; Tetraodon fluviatilis; Tetraodontidae; Amino Acid Sequence; Animals; Base Sequence; Caenorhabditis elegans; COS Cells; Drosophila; Gene Expression Regulation; Green Fluorescent Proteins; Humans; Luminescent Proteins; Molecular Sequence Data; Promoter Regions (Genetics); Recombinant Fusion Proteins; Ribosomal Proteins; Sequence Alignment; Sequence Analysis, Protein; Sequence Homology, Amino Acid; Species Specificity; Structure-Activity Relationship; Tetraodontiformes; Trans-Activation (Genetics); Zebrafish
Type
journal article
File(s)
Loading...
Thumbnail Image
Name

19.pdf

Size

564.98 KB

Format

Adobe PDF

Checksum

(MD5):4e90a58153ad03cbc2bfd7ff34a9bda9

臺大位居世界頂尖大學之列,為永久珍藏及向國際展現本校豐碩的研究成果及學術能量,圖書館整合機構典藏(NTUR)與學術庫(AH)不同功能平台,成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。

To permanently archive and promote researcher profiles and scholarly works, Library integrates the services of “NTU Repository” with “Academic Hub” to form NTU Scholars.

總館學科館員 (Main Library)
醫學圖書館學科館員 (Medical Library)
社會科學院辜振甫紀念圖書館學科館員 (Social Sciences Library)

開放取用是從使用者角度提升資訊取用性的社會運動,應用在學術研究上是透過將研究著作公開供使用者自由取閱,以促進學術傳播及因應期刊訂購費用逐年攀升。同時可加速研究發展、提升研究影響力,NTU Scholars即為本校的開放取用典藏(OA Archive)平台。(點選深入了解OA)

  • 請確認所上傳的全文是原創的內容,若該文件包含部分內容的版權非匯入者所有,或由第三方贊助與合作完成,請確認該版權所有者及第三方同意提供此授權。
    Please represent that the submission is your original work, and that you have the right to grant the rights to upload.
  • 若欲上傳已出版的全文電子檔,可使用Open policy finder網站查詢,以確認出版單位之版權政策。
    Please use Open policy finder to find a summary of permissions that are normally given as part of each publisher's copyright transfer agreement.
  • 網站簡介 (Quickstart Guide)
  • 使用手冊 (Instruction Manual)
  • 線上預約服務 (Booking Service)
  • 方案一:臺灣大學計算機中心帳號登入
    (With C&INC Email Account)
  • 方案二:ORCID帳號登入 (With ORCID)
  • 方案一:定期更新ORCID者,以ID匯入 (Search for identifier (ORCID))
  • 方案二:自行建檔 (Default mode Submission)
  • 方案三:學科館員協助匯入 (Email worklist to subject librarians)

Built with DSpace-CRIS software - Extension maintained and optimized by 4Science