https://scholars.lib.ntu.edu.tw/handle/123456789/143404
標題: | Zebrafish heparin-binding neurotrophic factor enhances neurite outgrowth during its development | 作者: | Chang, Ming-Huang Huang, Chang-Jen Hwang, Sheng-Ping L. Lu, I-Ching Lin, Chien-Ming Kuo, Tzong-Fu Chou, Chih-Ming |
關鍵字: | Heparin-binding neurotrophic factor; Neurite outgrowth; Neuron-specific promoter; PC12 cells; Zebrafish | 公開日期: | 2004 | 卷: | 321 | 期: | 2 | 起(迄)頁: | 502-509 | 來源出版物: | Biochemical and Biophysical Research Communications | 摘要: | Heparin-binding neurotrophic factor (HBNF) is a secreted heparin-binding protein containing highly basic and cysteine-rich amino acid residues. In this study, we cloned the full-length HBNF cDNA from zebrafish and determined its genomic structure by bioinformatics analysis. Zebrafish HBNF gene is composed of five exons and four introns spanning approximately 82kb. RT-PCR analysis revealed that zebrafish HBNF transcript was highly expressed in adult brain and intestine tissues while less in other tissues. During embryogenesis, zebrafish HBNF transcript was observed to be moderately expressed at earlier stages with a gradual decline. Higher expression level was observed after hatching and maintaining this level into adulthood. The overall amino acid sequence of zebrafish HBNF shows 60% identity to human HBNF, but with approximately 40% identity to other midkine proteins. Like mammalian homolog, zebrafish HBNF could induce significant neurite outgrowth in PC12 cells without NGF stimulation. In addition, zebrafish HBNF was able to enhance extensive neurite outgrowth in zebrafish embryos during embryogenesis. In summary, a feasible in vivo assay for neurite outgrowth was established in zebrafish. ? 2004 Elsevier Inc. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/162981 http://ntur.lib.ntu.edu.tw/bitstream/246246/162981/1/21.pdf https://www.scopus.com/inward/record.uri?eid=2-s2.0-3242761459&doi=10.1016%2fj.bbrc.2004.06.172&partnerID=40&md5=b8ea57e647f0f08c3fb6dbc88fadd4d1 |
DOI: | 10.1016/j.bbrc.2004.06.172 | SDG/關鍵字: | complementary DNA; midkine; nerve growth factor; pleiotrophin; zebrafish protein; carrier protein; cytokine; messenger RNA; pleiotrophin; animal cell; animal tissue; article; bioinformatics; brain; cell strain; controlled study; developmental stage; embryo; embryo development; exon; feasibility study; gene structure; genetic transcription; genome analysis; hatching; human versus animal comparison; in vitro study; intestine; intron; mammal; molecular cloning; nerve fiber growth; nonhuman; nucleotide sequence; priority journal; protein localization; rat; reverse transcription polymerase chain reaction; sequence homology; tissue distribution; zebra fish; amino acid sequence; animal; cell size; chemistry; cytology; drug effect; gene expression profiling; gene expression regulation; genetics; human; metabolism; molecular genetics; neurite; prenatal development; sequence alignment; zebra fish; Danio rerio; Mammalia; Amino Acid Sequence; Animals; Carrier Proteins; Cell Size; Cloning, Molecular; Cytokines; DNA, Complementary; Embryo; Gene Expression Profiling; Gene Expression Regulation, Developmental; Humans; Molecular Sequence Data; Neurites; PC12 Cells; Rats; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Sequence Alignment; Zebrafish |
顯示於: | 生化科學研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。