Research of CDR3 of TCR α Chain Repertoire in Human Thymus
Date Issued
2012
Date
2012
Author(s)
Tsai, Chung-Wei
Abstract
In theory, TCR is generated during T cell development in the thymus by a random process of assembling different gene segments, known as V(D)J recombination. The complementarity determining region (CDR) 3 is highly variable region of TCR. The studies of CDR3 from three thymic samples provide invaluable information. We address two topics. First, we investigated the recombination of human TCR variable (V) and junction (J) regions of α chain. Furthermore, the nucleotide usages, amino acid usages, a novel type of hybrid Jα TCR, and public TCR of three individuals were deciphered. Second, the thymic TCRα repertoire was assessed by experimental AmpliCot method and computer simulation program (MATLAB). Our first contribution is to exhibit the features of TCRα CDR3 and provide evidence in support of locus contraction mechanism of Vα-Jα recombination. Our second contribution is to explore the generation mechanism of public TCR. Importantly, the size of thymic TCRα repertoire is estimated to be 10^8 to 10^9.
Subjects
thymus
TCR
CDR3
T cell
repertoire
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
index.html
Size
23.27 KB
Format
HTML
Checksum
(MD5):3844208acc8d067d5732e7b719969260