Capillary electrophoresis using immobilized whole cells with overexpressed endothelin receptor for specific ligand screening
Journal
Electrophoresis
Journal Issue
25
Pages
1034-1041
Date Issued
2004
Date
2004
Author(s)
Abstract
A new capillary electrophoresis method using immobilized cells as the stationary phase has been developed. The power of this method is demonstrated by the separation and identification of endothelin antagonists on a capillary column coated by the transfected Chinese hamster ovary (CHO) cells with overexpressing endothelin receptors. The screening results are validated by functional assays suppressing the increase of intracellular calcium concentration induced by endothelin-1. Instead of making efforts in isolating protein receptors, the easily prepared whole-cell capillary column provides a superior tool on the basis of ligand/receptor affinity for a rapid screening of potent drug candidates from compound libraries.
Type
journal article
File(s)![Thumbnail Image]()
Loading...
Name
34.pdf
Size
141.31 KB
Format
Adobe PDF
Checksum
(MD5):f479ee13bd988ff9c849d59e7fa7eace