https://scholars.lib.ntu.edu.tw/handle/123456789/143770
Title: | Proteomic Analysis of Host Responses in HepG2 Cells during Dengue Virus Infection | Authors: | Pattanakitsakul, Sa-nga Rungrojcharoenkit, Kamonthip Kanlaya, Rattiyaporn Sinchaikul, Supachok Noisakran, Sansanee Chen, Shui-Tein Malasit, Prida Thongboonkerd, Visith |
Keywords: | Dengue; Host responses; Liver; Proteome; Proteomics; Virus | Issue Date: | 2007 | Start page/Pages: | 4592-4600 | Source: | Journal of Proteome Research | Abstract: | Dengue virus infection remains a public health problem worldwide. However, its pathogenic mechanisms and pathophysiology are still poorly understood. We performed proteomic analysis to evaluate early host responses (as indicated by altered proteins) in human target cells during dengue virus infection. HepG2 cells were infected with dengue virus serotype 2 (DEN-2) at multiplicity of infection (MOI) of 0.1, 0.5, and 1.0. Quantitative analyses of DEN-2 infection and cell death at 12, 24, and 48 h postinfection showed that the MOI of 1.0 with 24 h postinfection duration was the optimal condition to evaluate early host responses, as this condition provided the high %Infection (?80%), while %Cell death (?20%) was comparable to that of the mock-control cells. Proteins derived from the mock-control and DEN-2-infected cells were resolved by 2-D PAGE (n = 5 gels for each group) and visualized by SYPRO Ruby stain. Quantitative intensity analysis revealed 17 differentially expressed proteins, which were successfully identified by peptide mass fingerprinting. Most of these altered proteins were the key factors involved in transcription and translation processes. Further functional study on these altered proteins may lead to better understanding of the pathogenic mechanisms and host responses to dengue virus infection, and also to the identification of new therapeutic targets for dengue virus infection. ? 2007 American Chemical Society. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/163153 | DOI: | 10.1021/pr070366b | SDG/Keyword: | protein den 2; unclassified drug; virus protein; article; cell death; cell strain HepG2; controlled study; dengue; Dengue virus; human; human cell; mass spectrometry; priority journal; protein expression; proteomics; quantitative analysis; Cell Line, Tumor; Dengue; Dengue Virus; Hepatocytes; Humans; Proteomics; Dengue virus |
Appears in Collections: | 生化科學研究所 |
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