https://scholars.lib.ntu.edu.tw/handle/123456789/143949
標題: | Regulation of tristetraprolin during differentiation of 3T3-L1 preadipocytes | 作者: | Lin, Nien-Yi Lin, Chung-Tien Chen, Yu-Ling Chang, Ching-Jin |
關鍵字: | 3T3-L1; AU-rich element; Differentiation; mRNA degradation; Tristetraprolin | 公開日期: | 2007 | 卷: | 274 | 期: | 3 | 起(迄)頁: | 867-878 | 來源出版物: | FEBS Journal | 摘要: | Tristetraprolin is a zinc-finger-containing RNA-binding protein. Tristetraprolin binds to AU-rich elements of target mRNAs such as proto-oncogenes, cytokines and growth factors, and then induces mRNA rapid degradation. It was observed as an immediate-early gene that was induced in response to several kinds of stimulus, such as insulin and other growth factors and stimulators of innate immunity such as lipopolysaccharides. We observed that tristetraprolin was briefly expressed during a 1-8 h period after induction of differentiation in 3T3-L1 preadipocytes. Detailed analysis showed that tristetraprolin mRNA expression was stimulated by fetal bovine serum and differentiation inducers, and was followed by rapid degradation. The 3′UTR of tristetraprolin mRNAs contain adenine- and uridine-rich elements. Biochemical analyses using RNA pull-down, RNA immunoprecipitation and gel shift experiments demonstrated that adenine- and uridine-rich element-binding proteins, HuR and tristetraprolin itself, were associated with tristetraprolin adenine- and uridine-rich elements. Functional characterization confirmed that tristetraprolin negatively regulated its own expression. Thus, our results indicated that the tight autoregulation of tristetraprolin expression correlated with its critical functional role in 3T3-L1 differentiation. ? 2007 The Authors. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-33846420169&doi=10.1111%2fj.1742-4658.2007.05632.x&partnerID=40&md5=f084faad39baa5a4ee2a288ffd700ef6 | ISSN: | 1742464X | DOI: | 10.1111/j.1742-4658.2007.05632.x | SDG/關鍵字: | adenine; messenger RNA; tristetraprolin; uridine; 3' untranslated region; article; autoregulation; cell differentiation; controlled study; embryo; gene induction; mouse; nonhuman; observation; priority journal; proadipocyte; protein analysis; protein degradation; protein expression; protein function; regulatory mechanism; serum; upregulation; validation process; 3T3-L1 Cells; Adipocytes; Animals; Base Sequence; Blotting, Northern; Blotting, Western; Cell Differentiation; DNA-Binding Proteins; Electrophoretic Mobility Shift Assay; Gene Expression Regulation; Immediate-Early Proteins; Immunoprecipitation; Mice; Molecular Sequence Data; Protein Binding; Reverse Transcriptase Polymerase Chain Reaction; RNA Stability; RNA, Messenger; Time Factors; Tristetraprolin; Bovinae |
顯示於: | 生化科學研究所 |
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