https://scholars.lib.ntu.edu.tw/handle/123456789/143951
標題: | Modulation of immediate early gene expression by tristetraprolin in the differentiation of 3T3-L1 cells | 作者: | Lin, Nien-Yi Lin, Chung-Tien Chang, Ching-Jin |
關鍵字: | 3T3-L1; AU-rich element; Immediate early gene; MKP-1; Tristetraprolin | 公開日期: | 2008 | 起(迄)頁: | 69-74 | 來源出版物: | Biochemical and Biophysical Research Communications | 摘要: | Tristetraprolin (TTP) is a zinc-finger-containing AU-rich elements (ARE)-binding protein. AREs presented in the 3′untranslated region (UTR) of mRNAs from many proto-oncogenes, cytokines, and growth factors may be targets for regulation of messenger RNA stability. In this study, we observed that many immediate early genes (IEGs) were induced during the early differentiation of 3T3-L1 preadipocytes and their ARE-containing transcripts were degraded rapidly. Immunoprecipitation followed by RT-PCR analysis showed that two of IEG mRNAs, COX-2 (cyclooxygenase-2) and MKP-1 (mitogen-activated protein kinase phosphatase), were the target of TTP. Biotinylated MKP-1 AREs also could bring down TTP and the other ARE-binding protein HuR. RNA EMSA and competition assays showed that each of three AREs located in 3′UTR of MKP-1 mRNA has differential binding affinity to TTP. Sequence analysis of 3′UTR of IEG mRNAs suggested that TTP may prefer binding to UUAUUUAUU sequence. Taken together, our results implied that TTP may target specific ARE-containing IEGs' mRNAs such as COX-2 and MKP-1 mRNAs to modulate their expression post-transcriptionally. ? 2007 Elsevier Inc. All rights reserved. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/163286 | DOI: | 10.1016/j.bbrc.2007.10.119 |
顯示於: | 生化科學研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。