|Title:||利用飛行質譜儀作Mycobacterium avium之快速鑑定及區別||Authors:||何憲武||Keywords:||matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI/TOF-MS);Mycobacterium avium;identification||Issue Date:||2002||Publisher:||臺北市：國立臺灣大學醫學院醫學檢驗暨生物技術學系||Abstract:||
M. avium is a well known etiological agent for immuno-compromised patients,
especially for those with HIV infection. Rapid, accurate discrimination between M.
avium and other Mycobacterium species is essential for appropriate therapeutic
management and timely intervention for infection control. Identification and
speciation of M. avium is both time consuming and labor intensive by traditional
bacteriological methods. From our previous report [Yi et al., (1998) Use of
Fluorescein Labelled Antibody and Fluorescence Activated Cell Sorter for Rapid
Identification of Mycobacterium species. Biochem. Biophy. Res. Commu. 250:
403-408.], we have used Flow Cytometry for rapid identification of Mycobacterium
species. As we mentioned in the text, however, identification of Mycobacterium to the
species level can be achieved only depending on the availability of species-specific
In this article, a rapid method involving intact cell mass spectrometry (ICMS) is
presented that shows promise for identification, typing and discrimination of M.
avium from other Mycobacterium species. In ICMS, cells from a bacterial colony are
emulsified in a chemical matrix, added to a sample probe, dried and analysed by
matrix-assisted laser desorption ionization time-of-flight mass spectrometry
(MALDI-TOF-MS). This technique analyses proteins from intact and disrupted
bacteria yielding spectra consisting of 16 peaks from 5000 to 17500, which represent
the mass:charge (m:z) ratios. Each peak corresponds to a molecular fragment released
from the cell surface during laser desorption. Specimens can be prepared in a few
seconds from plate cultures and a spectrum can be obtained within 10 min. Our results
show that MALDI-TOF-MS is applicable for rapid identification and differentiation
of M. avium from, and thus shows its potential for clinical M. avium identification, or
for epidemiological studies.
|Appears in Collections:||醫學檢驗暨生物技術學系|
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