Repository logo
  • English
  • 中文
Log In
Have you forgotten your password?
  1. Home
  2. College of Medicine / 醫學院
  3. Clinical Laboratory Sciences and Medical Biotechnology / 醫學檢驗暨生物技術學系所
  4. 廢水生物處理系統生化指標與分析技術之建立
 
  • Details

廢水生物處理系統生化指標與分析技術之建立

Date Issued
2003
Date
2003
Author(s)
何憲武
DOI
912314B002295
URI
http://ntur.lib.ntu.edu.tw//handle/246246/27781
Abstract
Serratia marcescens swarming behavior is thermoregulated, which is characterized by continuous populational surface migration on rich swarming agar surface at 30°C, but not at 37°C. Here we report that environmental temperature shift and fatty acids (especially the saturated fatty acids, SFAs) act as the signal cues regulating S. marcescens swarming through an RssA-RssB two component signal transduction system. SFAs or temperature upshift specifically and negatively regulate S. marcescens swarming, but not swimming motility or the identified factors known to be involved in Serratia swarming, possibly through influencing pattern of cellular fatty acid profile. Fatty acid profile analysis strongly indicates that addition of SFAs at a constant temperature and temperature upshift may either directly or indirectly affect the signaling state of the RssA sensor protein by increasing the incorporation of the high-melting point fatty acids into membrane phospholipid, which subsequently inhibits S. marcescens swarming. The RssA-RssB two-component system mediates this response, as both partners are required to sense and transduce the signals. Expression of a fatty acid synthesis gene fabG and a hemolysin gene shlA is coordinately regulated with swarming by RssA-RssB. Biofilm formation, cell surface structure and extracellular polysaccharide are also affected in the rssA or rssB mutant. Similar phenomena of SFA inhibition are also observed in swarming regulation of Proteus mirabilis and Salmonella typhimurium. Thus, evidence suggests it may be common that membrane fluidity optimization through modulation of cellular fatty acid profile under different physiological culture conditions may subsequently affect two-component signaling pathway, which regulates the composition of cellular fatty acids, expression of virulence factor and multicellular swarming behaviours. This may provide an unidentified and common regulatory circuit for controlling swarming behaviour of not only S. marcescens, but also other swarming bacteria.
Subjects
Serratia marcescens, two-component system, thermosensor, swarming
motility, fatty acids.
Publisher
臺北市:國立臺灣大學醫學院醫學檢驗暨生物技術學系
Type
journal article
File(s)
Loading...
Thumbnail Image
Name

912314B002295.pdf

Size

247.89 KB

Format

Adobe PDF

Checksum

(MD5):278ddac9b6028629cff344ed45cda13a

臺大位居世界頂尖大學之列,為永久珍藏及向國際展現本校豐碩的研究成果及學術能量,圖書館整合機構典藏(NTUR)與學術庫(AH)不同功能平台,成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。

To permanently archive and promote researcher profiles and scholarly works, Library integrates the services of “NTU Repository” with “Academic Hub” to form NTU Scholars.

總館學科館員 (Main Library)
醫學圖書館學科館員 (Medical Library)
社會科學院辜振甫紀念圖書館學科館員 (Social Sciences Library)

開放取用是從使用者角度提升資訊取用性的社會運動,應用在學術研究上是透過將研究著作公開供使用者自由取閱,以促進學術傳播及因應期刊訂購費用逐年攀升。同時可加速研究發展、提升研究影響力,NTU Scholars即為本校的開放取用典藏(OA Archive)平台。(點選深入了解OA)

  • 請確認所上傳的全文是原創的內容,若該文件包含部分內容的版權非匯入者所有,或由第三方贊助與合作完成,請確認該版權所有者及第三方同意提供此授權。
    Please represent that the submission is your original work, and that you have the right to grant the rights to upload.
  • 若欲上傳已出版的全文電子檔,可使用Open policy finder網站查詢,以確認出版單位之版權政策。
    Please use Open policy finder to find a summary of permissions that are normally given as part of each publisher's copyright transfer agreement.
  • 網站簡介 (Quickstart Guide)
  • 使用手冊 (Instruction Manual)
  • 線上預約服務 (Booking Service)
  • 方案一:臺灣大學計算機中心帳號登入
    (With C&INC Email Account)
  • 方案二:ORCID帳號登入 (With ORCID)
  • 方案一:定期更新ORCID者,以ID匯入 (Search for identifier (ORCID))
  • 方案二:自行建檔 (Default mode Submission)
  • 方案三:學科館員協助匯入 (Email worklist to subject librarians)

Built with DSpace-CRIS software - Extension maintained and optimized by 4Science