https://scholars.lib.ntu.edu.tw/handle/123456789/146104
Title: | Analysis of the Steps Involved in Dengue Virus Entry into Host Cells | Authors: | HUNG, SHAN-LING CHEN, LI-KUANG KAO, CHUAN-LIANG KING, CHWAN-CHUEN |
Keywords: | Dengue;Virus Entry;VIrus Attachment;Pathogenesis;Monoclonal Antibody;Mosquito | Issue Date: | 1999 | Journal Volume: | v.257 | Journal Issue: | n.1 | Start page/Pages: | 156-167 | Source: | VIROLOGY | Abstract: | The initial steps of dengue viral entry have been divided into adsorption and penetration using acid glycine treatment to inactivate extracellular virus after attachment to baby hamster kidney (BHK) cells but prior to penetration. First, we showed that virus infection was accomplished within 2 h after adsorption. Second, the assay was used to examine the properties of dengue envelope E protein-specific monoclonal antibodies ( MAbs), lectins, and heparin. We found that three MAbs, 17-2, 46-9, and 51- 3, may neutralize dengue 2 virus ( DEN-2) through inhibition of not only viral attachment but also of penetration. However, one MAb, 56-3.1, interfered specifically with attachment. Therefore, the functional domains of E protein involved in attachment and penetration may be different. Moreover, studies with lectins indicated that carbohydrates, especially alpha-mannose residues, present on the virion glycoproteins may contribute to binding and penetration of the virus into BHK and mosquito C 6/36 cells. Finally, virus |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/105259 |
Appears in Collections: | 醫學檢驗暨生物技術學系 |
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