https://scholars.lib.ntu.edu.tw/handle/123456789/146373
標題: | Fas-Ligand-Expressing Adenovirus-Transfected Dendritic Cells Decrease Allergen-Specific T Cells and Airway Inflammation in a Murine Model of Asthma | 作者: | CHUANG, YA-HUI SUEN, JAU-LING CHIANG, BOR-LUEN |
關鍵字: | OVA-specific T cells;Adenoviral vector;CD95 ligand;Apoptosis;Gene therapy | 公開日期: | 2006 | 卷: | v.84 | 期: | n.7 | 起(迄)頁: | 595-603 | 來源出版物: | JOURNAL OF MOLECULAR MEDICINE-JMM | 摘要: | T cells expressing a type-2 T helper profile of cytokines ( Th2 cells) have been demonstrated to play an important role in the initiation and progression of allergic asthma, and it is well known that Fas ligand (FasL ) induces apoptosis when bound to its receptor, Fas. In the present study, we examined the possibility of modulating asthma manifestations by dendritic cells (DCs) genetically engineered to express FasL (DC-FasL), which could deliver a death signal to T cells in an antigen-specific manner. The delivery of DC-FasL into ovalbumin (OVA)-immunized allergic mice decreased the airway hyper-responsiveness(AHR). Moreover, we established a mouse model of airway inflammation by using an adoptive transfer of Th2 cells derived from ovalbumin T cell receptor transgenic mice to study the effect of DC-FasL on airway reactivity. The administration of DC-FasL in Th2-cell- induced allergic mice had significantly decreased AHR, airway inflammation, and IL-4, IL-5 and IL-13 production. Furthermore, the numbers of OVA-specific T cells were decreased in the lung of mice receiving DC-FasL. These results demonstrate that FasL-expressing dendritic cells might be applied for the modulation of allergic responses. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/94566 |
顯示於: | 醫學檢驗暨生物技術學系 |
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