|Title:||Human Tlr3 Recognizes Dengue Virus and Modulates Viral Replication in Vitro||Authors:||TSAI, YI-TING
|Issue Date:||2009||Journal Volume:||v.11||Journal Issue:||n.4||Start page/Pages:||604-615||Source:||CELLULAR MICROBIOLOGY||Abstract:||
The elicitation of large amount inflammatory cytokine in serum has been developed as the cause of the plasma leakage in dengue fever (DF)/dengue haemorrhagic fever (DHF) infection. Virus recognition in innate immunity is the key. The Toll-like receptors (TLRs) play an important role in pathogen recognition towards cytokine induction among several viruses; however, the role of TLRs on innate immune recognition against DENV remains unclear. This study aims at the interaction between dengue virus ( DENV) and human TLRs at the incipient stage of infection in vitro. Our experiment reveals that stably expression of TLR3, 7, 8 on HEK293 enables IL-8 secretion after DENV recognition. By the model of human monocytic cells U937, we demonstrated the trigger of IL-8 after viral recognition of human monocytic cell is primary through TLR3 following endosomal acidification. Silencing of TLR3 in U937 cells significantly blocks the DENV-induced IL-8 production. Besides, the interaction is further corroborated by colocalization of TLR3 and DENV RNA upon DENV internalization. Furthermore, in this study we found the expression of TLR 3 can mediate strong IFN-alpha/ beta release and inhibit DENV viral replication significantly, thus limit the cytopathic effect.
|Appears in Collections:||醫學檢驗暨生物技術學系|
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