https://scholars.lib.ntu.edu.tw/handle/123456789/149810
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor | 林啟萬 | en |
dc.contributor | 臺灣大學:電機工程學研究所 | zh_TW |
dc.contributor.author | 李振戎 | zh |
dc.contributor.author | Lee, Cheng-Jung | en |
dc.creator | 李振戎 | zh |
dc.creator | Lee, Cheng-Jung | en |
dc.date | 2006 | en |
dc.date.accessioned | 2007-11-26T06:56:57Z | - |
dc.date.accessioned | 2018-07-06T11:07:05Z | - |
dc.date.available | 2007-11-26T06:56:57Z | - |
dc.date.available | 2018-07-06T11:07:05Z | - |
dc.date.issued | 2006 | - |
dc.identifier | en-US | en |
dc.identifier.uri | http://ntur.lib.ntu.edu.tw//handle/246246/53523 | - |
dc.description.abstract | 本文以發展可攜式及高通量生物分子感測平台為目標,分別針對其感測理論、電路設計、及分析演算法作初步的探討。在相關的電路測試及偵測實驗中,已成功的利用CMOS影像感測器(CIS)、可程式化邏輯閘(FPGA)仿真模擬電路板與顯示電路,量測到半徑不同之奈米金粒子所發出之散射光訊號,對本實驗室所研發之偵測系統架構做了初步的驗證。 利用奈米粒子作為標記的生物分子可做為生物晶片的影像掃瞄反應樣本,經由偵測生物晶片表面奈米粒子散射光線的強度,可以分辨出相關基因或蛋白質晶片探針與標的物之雜交結合效率。奈米粒子有多種的偵測方式在近來的研究中被提出,在微小與晶片化上有具體的成果,並證實其可行性與正確性。在此將針對其中光偵測的方式做討論,奈米粒子散射光偵測系統之優勢為高通量並且與之前所廣泛使用的螢光分子檢測方式較為類似;不同於以雙波段雷射光激發螢光分子,再利用光電倍增管接收訊號的方式,奈米粒子標記的感測原理是經由漸逝波激發表面電漿共振,能夠以透射、反射與散射方式觀察到由金屬奈米粒子所發出的光訊號 [6]。 目前在實驗中所發展出的影像擷取系統,使用CMOS影像感測器模組做為光感測元件,接收奈米粒子所散射出來的光訊號,將光線強度轉換成數位信號之後,傳輸到可程式邏輯閘,以便於進行計算與顯示的處理。為便於分析影像感測器應用於生物晶片應用上的特性,另外一組實驗設計則是利用市售的網路攝影機,規格為解析度30萬畫素的CMOS影像感測器,利用USB傳輸到個人電腦,並使用軟體做分析。實驗成果目前已經能夠經由光線強度與色彩比值針對不同的奈米金粒子大小做辨別。 | zh_TW |
dc.description.abstract | The goal of this study is to develop a high throughput and portable biomolecules detecting system. Discussions of the detecting theory, circuit design, and the analysis algorithm are disclosed respectively. In related circuit tests and detecting experiments, the measurement of the scattering optical signals which emitted from various sizes of gold nanoparticles through CMOS image sensor (CIS), field programmable gate array (FPGA) emulation board, and video display circuit system had successfully achieved. Thus there is the preliminary verification for this detection system structure. Biomolecules labeled with nanoparticles are able to be used as the reacted samples for biochip image scanning. By detecting the light intensity of nanoparticles scattering on the surface of biochip, it can distinguish the hybridization efficiency of target and probe regarding to relative gene or protein. Several detection methods have been provided in recent studies, to minimize and to integrate system on a single chip already has been implemented and the feasibility and validity have been proven. This study focuses on the light detection approach. The nanoparticles scattering light detection has the advantage of high throughput and is compatible with conventional fluorescence dye labeling method; however the detection is not excited by laser beam and received by photo multiplier tube. The optical signal comes from the evanescent wave induced surface plasmon resonance and observing is allowed by means of transmission, reflection and scattering [6]. The system utilized to capture image includes a CIS module as the light receiving device. After catching the light signal generated by nanoparticles scattering, CIS transfers the light intensity to digital signals and transmits to FPGA to do the processing of calculation and display. Another experimental setup uses a commercial web camera to analyze the characteristics of image sensor in biochip application. The specification of web camera is 300K pixel resolution and this camera connects to personal computer by USB interface. The image analysis is done by software and already can identify the differences between various size nanoparticles through light intensity and color ratio. | en |
dc.description.tableofcontents | 中文摘要 I Abstract III Preface V Chapter 1 Theory Background 1 1-1 SOC System 1 1-1-1 CMOS-based Biosensor Arrays 2 1-1-2 Nanoparticle-based Optical Detection 2 1-2 Surface Plasmon Resonance 3 1-2-1 Biochips in Drug Development 5 1-2-2 SPR Application in Immunogenicity 5 1-2-3 Localize SPR and Light Scattering 6 1-3 Microarray 7 1-4 Nanoparticles 9 Chapter 2 Material and Method 12 2-1 Chip Preparation 12 2-1-1 Light Source 12 2-1-2 Nanoparticle Solution 12 2-1-3 Microarray Printer 13 2-2 Measurement System Design 14 2-2-1 Methodology 14 2-2-2 Timing chart of CMOS Image Sensor 15 2-2-3 Comparison between CCD and CIS 16 2-3 FPGA Circuit Design 17 2-3-1 Data Path 18 2-3-2 Control Path 19 2-3-3 Operating Mechanism 19 Chapter 3 Experiment and Result 21 3-1 Experiment Setup 21 3-1-1 Measuring Device Setup 21 3-1-2 Image Capturing 21 3-2 Profile Intensity and Histogram Comparison 23 3-3 Image Analysis 29 Chapter 4 Conclusion and Future Work 31 4-1. Conclusion 31 4-1-1 Connection to DNA Microarray 32 4-1-2 Improvement of the sensor 33 4-2. Future Work 34 4-2-1 Experimental Procedures Design 34 4-2-2 IC Testing Equipments 35 4-2-3 Biological Applications 36 Reference 38 | zh_TW |
dc.format.extent | 1948785 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language | en-US | en |
dc.language.iso | en_US | - |
dc.subject | 奈米粒子 | en |
dc.subject | 散射效應 | en |
dc.subject | 表面電漿共振 | en |
dc.subject | 可程式化邏輯閘 | en |
dc.subject | CMOS影像感測器 | en |
dc.subject | Nanoparticle | en |
dc.subject | Scattering | en |
dc.subject | Surface Plasmon Resonance | en |
dc.subject | Field Programmable Gate Array | en |
dc.subject | CMOS Image Sensor | en |
dc.title | 奈米粒子散射與表面電漿共振影像偵測系統晶片 | zh |
dc.title | A SOC Image Detection System for Nanoparticle Scattering and Surface Plasmon Resonance | en |
dc.type | thesis | en |
dc.identifier.uri.fulltext | http://ntur.lib.ntu.edu.tw/bitstream/246246/53523/1/ntu-95-P93921012-1.pdf | - |
dc.relation.reference | [1] Assay and screening methods for bioactive substances based on cellular signaling pathways, Reviews in Molecular Biotechnology Volume 82, Issue 4, 357-370, February 2002. [2] A. M. Dudley, J. Aach, M. A. Steffen, and G. M. Church. Measuring absolute expression with microarrays using a calibrated reference sample and an extended signal intensity range. PNAS 99:7554-9, 2002. [3] D. A. Stuart, A. J. Haes, C. R. Yonzon, E. M. Hicks, and R. P. Van Duyne, Biological applications of localised surface plasmonic phenomenae, IEE Proc.- Nanobiotechnol.,152, 13-32, 2005. [4] A. Frigessi, M. van de Wiel, M. Holden, I. Glad, and H. Lyng, Model-based estimation of transcript concentrations from spotted microarray data. NR research report 999, ISBN: 82-539-0507-6, 2004. [5] G. Festag, A. Steinbrück, A. Wolff, A. Csaki, R. Möller and W. Fritzsche: Optimization of Gold Nanoparticle-Based DNA Detection for Microarrays. Journal of Fluorescence 15, 161-170, 2005. [6] Hao-Ran Lee, Chii-Wann Lin, Biochip Array Detection based on Plasmon Resonance and Evanescent Wave Induced Scattering, thesis, 2005. [7] E. Hutter, J. H. Fendler, Exploitation of localized surface plasmon resonance. Adv Mater, 16:1685-1706, 2004. [8] http://homepages.wmich.edu/~sobare/index.htm [9] http://www.innovations-report.de/ [10] I. Takayanagi, M. Shirakawa, S. Iversen, J. Moholt, J. Nakamura and E. Fossum, A 1 ¼ inch 8.3M Pixel Digital Output CMOS APS for UDTV Application, ISSCC 2003, San Francisco, CA, 2003. [11] J. Homola, Present and Future of Surface Plasmon Resonance Biosensors, Analytical and Bioanalytical Chemistry, 377, 528-539, 2003. [12] J. Yguerabide and E. E. Yguerabide, Light-scattering submicroscopic particles as highly fluorescent analogs and their use as tracer labels in clinical and biological applications - I.Theory, Anal Biochem, vol. 262, pp. 137-56, 1998. [13] K. Mitani, M. Sugawara and F. Okano, Experimental Ultrahigh-Definition Color Camera System with Three 8M pixel CCDs, SMPTE Journal, April 2002. [14] Kadir Aslan, Joseph R. Lakowicz and Chris D. Geddes, Plasmon light scattering in Biology and Medicine: New sensing approaches, visions and perspectives, Current Opinions in Chemical Biology, 9, 538-544, 2005. [15] Lester J. Kozlowski, CMOS vs. CCD Changing Technology to Suit HDTV Broadcast, Rockwell Scientific 1049 Camino Dos Rios Thousand Oaks, CA 91360. [16] H. Lyng, A. Badiee, D. H. Svendsrud, E. Hovig, O. Myklebost, and T. Stokke, Profound influence of scanner characteristics on gene expression ratios: analysis and procedure for correction. BMC Genomics, 5, 10, 2004. [17] M.-H. Tsai, H. Yan, G. V. R. Chandramouli, S. Zhao, D. Coffin, C. N. Coleman, J. B. Mitchell, and E. Y. Chuang, Evaluation of Hybridization Conditions for Spotted Oligonucleotide Based DNA Microarrays, Mol. Biotechnol, 29, 221-224, 2005. [18] M. Schienle, A. Frey, et. Al., A fully electronic DNA sensor with 128 positions and in-pixel A/D conversion, in IEEE ISSCC Dig. Tech. Papers, pp. 220–221, 2004. [19] Mark Thoreson, Sayeef Salahuddin, Neophytos Neophytou, Plasmonic Nanostructures in Sensing Molecules. [20] Mark Schena, Microarray Analysis. [21] Michael B. Sinclair, Jerilyn A. Timlin, David M. Haaland, and Margaret Werner-Washburne, Design, construction, characterization, and application of a hyperspectral microarray scanner. Applied Optics, Vol. 43 Issue 10 Page 2079-2088, April 2004. [22] C. Romualdi, S. Trevisan, B. Celegato, G.. Costa, G. Lanfranchi, Improved detection of differentially expressed genes in microarray experiments through multiple scanning and image integration, Nucleic Acids Res. 31: e149, 2003. [23] Sandrine Dudoit, Pre-processing in DNA microarray experiments. [24] J.J. Storhoff, S.S. Marla, P. Bao, S. Hagenow, H. Mehta, A. Lucas, V. Garimella, T. Patno, W. Buckingham, W. Cork, et al. Gold nanoparticle-based detection of genomic DNA targets on microarrays using a novel optical detection system, Biosens. Bioelectron, 19:875–883, 2004. [25] T. A. Taton, C. A. Mirkin, and R. L. Letsinger, "Scanometric DNA array detection with nanoparticle probes", Science, 289, pp. 1757-60, 2000. [26] W. Fritzsche, A. Csaki, R. Möller: Nanoparticle-Based Optical Detection of Molecular Interactions for DNA-Chip Technology. SPIE 4626 (2002), 17-22 [27] U. Kreibig, M. Vollmer, Optical Properties of Metal Clusters, Springer, Berlin, 1995 | en |
item.openairecristype | http://purl.org/coar/resource_type/c_46ec | - |
item.openairetype | thesis | - |
item.languageiso639-1 | en_US | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.fulltext | with fulltext | - |
顯示於: | 電機工程學系 |
檔案 | 描述 | 大小 | 格式 | |
---|---|---|---|---|
ntu-95-P93921012-1.pdf | 23.31 kB | Adobe PDF | 檢視/開啟 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。