The study of Growth arrest-specific 7 Involves in Cyclin-dependent kinase 5/p35 Kinase complex
Date Issued
2005
Date
2005
Author(s)
Chung, Tai-Lien
DOI
en-US
Abstract
Cyclin-dependent kinase 5 (Cdk5), a serine/threonine kinase, which is dependent on association with its neuronal specific activator, p35, is required for proper development of a mouse brain. Recently, the Cdk5-p35 kinase has been detected in the Golgi apparatus and is a factor in the regulation of membrane traffic, although the action of Cdk5-p35 in the Golgi apparatus is largely unclear. The growth arrest-specific 7 (Gas7) is identified in G0 phase and expressed preferentially in the brain. Gas7 C’-terminal Binding Protein, CBP1, is highly homologous with p35-interacting protein, but its biological function is not understood. Moreover, Gas7 is associated with actin assembly that is responsible for protein transport in Golgi apparatus. This study will determine the complex formation of Gas7, CBP1, p35 and Cdk5 tetra-complex, and their roles in Golgi architecture and Golgi-mediated cellular functions including vesicle transport. In protein-protein relationships, no direct interaction been detected between Gas7 and p35/Cdk5 kinase without mediation by CBP1. Preliminary results indicate that the pattern of Golgi distribution by analyzing specific Golgi markers of sucrose gradient prepared from Gas7 wild-type developing mouse brains reveals Gas7 and p35 are predominantly expressed in Golgi-enriched fractions. Moreover, the tetra-complex formation of Gas7, actins and Cdk5/p35, exists in Golgi-enriched microsomal fractions purified from the developing mouse brain. In the VSVG-ts045-GFP transport assay, CBP1 delays the vesicle transportation from the ER to the Golgi apparatus. Collectively, these results indicate that CBP1, a key component for Gas7 and p35/CDK5 complex formation, is not only predominantly expressed in the Golgi complex but also plays a role in protein trafficking.
Subjects
生長休止基因七
Gas7
Type
other
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