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  4. Expression of Stathmin in Urothelial carcinoma
 
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Expression of Stathmin in Urothelial carcinoma

Date Issued
2005
Date
2005
Author(s)
Huang, Kuo-How
DOI
zh-TW
URI
http://ntur.lib.ntu.edu.tw//handle/246246/51358
Abstract
Purpose: Stathmin is a ubiquitous cytosolic phosphoprotein that regulates dynamics of microtubule polymerization. The activity of stathmin is regulated via phosphorylation and dephosphrylation by various protein kinase. Stathmin has been proved to play a role in human cancers. including lung, breast, ovary, leukemia, lymphoma, neuroblastoma etc. However, no relevant data in urothelial carcinoma was reported. In this way, we hope to analyze the expression of stathmin in human urothelial carcinoma (UC) cell lines and tissue samples. We also hope to study the potential of stathmin as predictor in tumor behavior and response to chemotherapy. The differential expression of Ser-16 phosphorylated stathmin (s-16 stathmin) and total stathmin was also studied. Materials and methods: We collected 3 urothelial cell lines NTUB1 (high grade urothelial carcinoma), NTUB1/P (cisplatin resistant), NTUB1/T (taxol resistant) and checked the total stathmin and s-16 stathmin expression by immunofluorescence staining and western blot analysis. Besides, we collected 85 urothelial carcinoma surgical specimens and analyzed the stathmin expression by immunohistochemical staining in paraffin-embedded cancer tissue samples. Correlation between stathmin expression and clinicopathologic features, such as age, sex, primary tumor site, black foot disease endemic area residency, chronic renal insufficiency, tumor grading and pathological staging, overall survival, disease free survival and response to TP-HDFL regimen (paclitaxel, cisplatin, high dose fluorouracil, and leucovorin) were examined. Results: Total stathmin and s-16 stathmin were both highly expressed in 3 cell lines without significant quantitative diffenence. The expression present in both cytoplasm and nucleus was proved by nuclear cytoplasmic fractionation and western blot analysis. Stathmin was also expressed in all stages of cell cycle. In urothelial carcinoma tissue, s-16 stathmin expression was significantly associated with tumor grading( p=0.022) and pathological staging (p=0.006). The response to TP-HDFL in metastatic invasive urothelial carcinoma was significantly correlated with s-16 stathmin expression. There was no difference in overall and progression free survival in reference to the expression of stathmin. Total stathmin expression was significantly associated with pathological staging. Total stathmin expressin is significantly related to the risk of superficial urothelial carcinoma progression to invasive disease. Conclusions: In our preliminary data, we observed high expression rate of stathmin in urothelial carcinoma cell lines and tumor tissue. Stathmin expression is related to disease status and tumor behavior of urothelial carcinoma. Further study will be necessary to determine the role of stathmin in urothelial carcinoma and the potential application for prediction of chemotherapy response.
Subjects
微小管
細胞週期
太平洋紫杉醇
microtubule
cell cycle
Paclitaxel
SDGs

[SDGs]SDG3

Type
other
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ntu-94-P92448007-1.pdf

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