Repository logo
  • English
  • 中文
Log In
Have you forgotten your password?
  1. Home
  2. College of Medicine / 醫學院
  3. Molecular Medicine / 分子醫學研究所
  4. TRB2交互作用蛋白之功能研究
 
  • Details

TRB2交互作用蛋白之功能研究

Functional characterization of TRB2 interacting proteins

Date Issued
2007
Date
2007
Author(s)
Hung, Chien-Min
DOI
en-US
URI
http://ntur.lib.ntu.edu.tw//handle/246246/51372
Abstract
Tribbles, a serine/threoine protein kinase family member, has been shown to coordinate cell proliferation, migration and morphogenesis during the development of Drosophila embryo. Although Tribbles are highly conserved throughout the evolution, the physiological function of mammalian orthologs is largely unknown. Recently, our laboratory has demonstrated that human Tribbles ortholog 2 (TRB2) is involved in GM-CSF withdrawal-induced apoptosis of TF-1 cells, a human erytholeukemia cell line. TRB2 mRNA is highly induced upon removal of GM-CSF, and ectopic expression of TRB2 promotes apoptosis of cells mainly of the hematopoietic origin. However, the underlying mechanism of TRB2-induced apoptosis needs to be further elucidated. For this purpose, we firstly report here that the putative kinase activity is not required for the pro-apoptotic function of TRB2, and its protein level is regulated through the proteasome- dependent pathway. Second, a yeast two-hybrid screen was previously applied and several candidate genes were identified. In this dissertation, we further characterized the interactions of three of these proteins, MNK2b, γ-Parvin and UBC9, with TRB2 in vitro and in mammalian cells. The interaction of TRB2 with MNK2b or γ-Parvin could be demonstrated in GST pull-down experiments. The co-immunoprecipiation and the mammalian two-hybrid assay were further used to confirm TRB2 associates with γ-Parvin in cells. In contrast, the interaction between TRB2 and UBC9 was not detected both in vitro and in vivo. Last, we explored the biological effect of these interactions on apoptosis and found that MNK2b and γ-Parvin partially interfere with the pro-apoptotic function of TRB2. Taken together, our data suggest that γ-Parvin is likely a physiological binding protein of TRB2 and may play a role in TRB2-induced apoptosis.
Subjects
細胞凋亡
細胞激素剝奪
Tribbles
MNK2b
γ-Parvin
UBC9
Apoptosis
Cytokine deprivation
Type
other
File(s)
Loading...
Thumbnail Image
Name

ntu-96-R94448009-1.pdf

Size

23.31 KB

Format

Adobe PDF

Checksum

(MD5):c04ffdb773fc6fcda7ffaabdc03bb528

臺大位居世界頂尖大學之列,為永久珍藏及向國際展現本校豐碩的研究成果及學術能量,圖書館整合機構典藏(NTUR)與學術庫(AH)不同功能平台,成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。

To permanently archive and promote researcher profiles and scholarly works, Library integrates the services of “NTU Repository” with “Academic Hub” to form NTU Scholars.

總館學科館員 (Main Library)
醫學圖書館學科館員 (Medical Library)
社會科學院辜振甫紀念圖書館學科館員 (Social Sciences Library)

開放取用是從使用者角度提升資訊取用性的社會運動,應用在學術研究上是透過將研究著作公開供使用者自由取閱,以促進學術傳播及因應期刊訂購費用逐年攀升。同時可加速研究發展、提升研究影響力,NTU Scholars即為本校的開放取用典藏(OA Archive)平台。(點選深入了解OA)

  • 請確認所上傳的全文是原創的內容,若該文件包含部分內容的版權非匯入者所有,或由第三方贊助與合作完成,請確認該版權所有者及第三方同意提供此授權。
    Please represent that the submission is your original work, and that you have the right to grant the rights to upload.
  • 若欲上傳已出版的全文電子檔,可使用Open policy finder網站查詢,以確認出版單位之版權政策。
    Please use Open policy finder to find a summary of permissions that are normally given as part of each publisher's copyright transfer agreement.
  • 網站簡介 (Quickstart Guide)
  • 使用手冊 (Instruction Manual)
  • 線上預約服務 (Booking Service)
  • 方案一:臺灣大學計算機中心帳號登入
    (With C&INC Email Account)
  • 方案二:ORCID帳號登入 (With ORCID)
  • 方案一:定期更新ORCID者,以ID匯入 (Search for identifier (ORCID))
  • 方案二:自行建檔 (Default mode Submission)
  • 方案三:學科館員協助匯入 (Email worklist to subject librarians)

Built with DSpace-CRIS software - Extension maintained and optimized by 4Science