狼瘡病人血清中抗中性白血球抗體的免疫活性,對應抗原的鑑定,及臨床應用(2/3)
Date Issued
2003-05-21
Date
2003-05-21
Author(s)
余家利
DOI
912314B002129
Abstract
Decreased number and impaired functions of polymorphonuclear
neutrophils (PMN) due to the presence of anti-PMN autoantibodies in the
serum render patients with systemic lupus erythematosus susceptible to
bacterial infections. However, the cognate antigens and pathological
mechanisms of anti-PMN autoantibodies in SLE are rarely reported in the
literature. In this study, we found approximately 20% of SLE sera contained
anti-PMN autoantibodies detected by human PMN-coated cellular ELISA. A
membrane protein with molecular weight of 50 kDa was identified as the
cognate antigen of anti-PMN in Western blot after membrane-biotinylation
and strptavidin column elution. The 50kDa molecule was proved to be SSB/La
after immunoscreening, molecular cloning and nucleotide sequencing of the
gene from the human leukocyte cDNA library. Human anti-SSB/La
autoantibodies purified from active SLE sera passing through the recombinant
SSB/La conjugated Sepharose 4B affinity column could bound and penetrate
into normal human PMN. Functional analysis revealed that the anti-SSB/La
autoantibodies exerted a number of potent effects on human PMN, including
suppressed phagocytosis, accelerated apoptosis and enhanced IL-8 production.
These in vitro results suggest that anti-SSB/La is one of the anti-PMN
autoantibodies capable of penetrating into PMN and responsible for
neutropenia and functional impairment of PMN in patients with SLE.
Subjects
anti-neutrophil autoantibodies
bacter ial infection
neutropenia
systemic lupus erythematosus
SSB/La
Publisher
臺北市：國立臺灣大學醫學院分子醫學研究所
Type
journal article
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