DC Field | Value | Language |
dc.contributor.author | 余家利 | zh-TW |
dc.creator | 余家利 | zh_TW |
dc.creator | Yu, Chia-Li | en |
dc.date | 2003-05-21 | zh_TW |
dc.date.accessioned | 2006-07-26T03:26:34Z | - |
dc.date.accessioned | 2018-07-09T01:13:33Z | - |
dc.date.available | 2006-07-26T03:26:34Z | - |
dc.date.available | 2018-07-09T01:13:33Z | - |
dc.date.issued | 2003-05-21 | - |
dc.identifier | 912314B002129 | zh_TW |
dc.identifier.uri | http://ntur.lib.ntu.edu.tw//handle/246246/23774 | - |
dc.description.abstract | 由於血清中有抗中性白血球抗體(anti-PMN antibodies)的存在,引起中
性球數目的減少以及功能上的缺陷,致使全身性紅斑狼瘡病人(SLE)易受
細菌的感染。然而SLE 病人血清中的anti-PMN 的對應抗原以及病理機轉
為何?在文獻上很少報告。在本研究中,我們以PMN cellular ELISA 法發
現約有20%的SLE 病人血清中含有anti-PMN 抗體。我們也發現在PMN
細胞表面的50kDa 分子為anti-PMN 抗體的對應抗原之一。以免疫抗體篩
選法,基因選殖及核甘酸序列分析證實50kDa 蛋白是為SSB/La 分子。我
們將活動性SLE病人的血清流經SSB/La-結合的親和性層析管柱純化出抗
SSB/La 抗體。本抗體會結合於PMN 細胞表面而後貫穿進入細胞內,引起
很多的生物活性,包括抑制吞食作用、加速細胞凋亡、以及增加IL-8 的
產生。這些生體外的結果顯示anti-SSB/La 抗體乃屬anti-PMN
autoantibodies 的一種,會進入白血球內引起SLE 病人的白血球減少及機
能異常。 | zh_TW |
dc.description.abstract | Decreased number and impaired functions of polymorphonuclear
neutrophils (PMN) due to the presence of anti-PMN autoantibodies in the
serum render patients with systemic lupus erythematosus susceptible to
bacterial infections. However, the cognate antigens and pathological
mechanisms of anti-PMN autoantibodies in SLE are rarely reported in the
literature. In this study, we found approximately 20% of SLE sera contained
anti-PMN autoantibodies detected by human PMN-coated cellular ELISA. A
membrane protein with molecular weight of 50 kDa was identified as the
cognate antigen of anti-PMN in Western blot after membrane-biotinylation
and strptavidin column elution. The 50kDa molecule was proved to be SSB/La
after immunoscreening, molecular cloning and nucleotide sequencing of the
gene from the human leukocyte cDNA library. Human anti-SSB/La
autoantibodies purified from active SLE sera passing through the recombinant
SSB/La conjugated Sepharose 4B affinity column could bound and penetrate
into normal human PMN. Functional analysis revealed that the anti-SSB/La
autoantibodies exerted a number of potent effects on human PMN, including
suppressed phagocytosis, accelerated apoptosis and enhanced IL-8 production.
These in vitro results suggest that anti-SSB/La is one of the anti-PMN
autoantibodies capable of penetrating into PMN and responsible for
neutropenia and functional impairment of PMN in patients with SLE. | en |
dc.format | application/pdf | zh_TW |
dc.format.extent | 98638 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language | zh-TW | zh_TW |
dc.language.iso | zh_TW | - |
dc.publisher | 臺北市:國立臺灣大學醫學院分子醫學研究所 | zh_TW |
dc.rights | 國立臺灣大學醫學院分子醫學研究所 | zh_TW |
dc.subject | anti-neutrophil autoantibodies | en |
dc.subject | bacter ial infection | en |
dc.subject | neutropenia | en |
dc.subject | systemic lupus erythematosus | en |
dc.subject | SSB/La | en |
dc.subject | 抗中性球自體抗體 | zh_TW |
dc.subject | 細菌性感染 | zh_TW |
dc.subject | 中性球減少症 | zh_TW |
dc.subject | 全身性紅斑狼瘡 | zh_TW |
dc.subject | 抗SSB/La 抗體 | zh_TW |
dc.title | 狼瘡病人血清中抗中性白血球抗體的免疫活性,對應抗原的鑑定,及臨床應用(2/3) | zh_TW |
dc.type | journal article | en |
dc.identifier.uri.fulltext | http://ntur.lib.ntu.edu.tw/bitstream/246246/23774/1/912314B002129.pdf | - |
dc.coverage | 計畫年度:91;起迄日期:2002-08-01/2003-07-31 | zh_TW |
item.languageiso639-1 | zh_TW | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
item.fulltext | with fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
Appears in Collections: | 分子醫學研究所
|