DNA-dependent protein kinase在細胞有絲分裂的功能(2/3)
Date Issued
2005-05-30
Date
2005-05-30
Author(s)
呂勝春
DOI
932320B002109
Abstract
Proteins of the poly(ADP-ribose) polymerase (PARP) family play wide array of
functions, covering virtually every aspect of DNA metabolism and function, most
notably with the response to DNA damage, transcription and the maintenance of
genomic stability. Here we report the identification and characterization of a novel
PARP family member, PARP10 (FLJ14464 or hypothetical protein LOC84875).
Over-expression of PARP10 results in loss of cell viability, while knockdown by
siRNA leads to delayed G1 progression and concomitant cell death. PARP10 exists
in both cytoplasm and nucleus, only that in nucleolus is specifically phosphorylated
by Cdk through late-G1 to S phase. In an in vitro assay, the enzymatic activity of
PARP10 depends on the Cdk phosphorylation. The phosphorylation is shut down in
G2, and re-appeared from prometaphase to cytokinesis in the nucleolar organizing
regions. The phosphoryated PARP10 is completely absent in growth-arrested cells
and may serve as a marker for proliferating cells. These results suggest that PARP10
functions in cell proliferation.
Subjects
PARP10
Cdk
cell cycle progression
Publisher
臺北市：國立臺灣大學醫學院分子醫學研究所
Type
journal article
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