https://scholars.lib.ntu.edu.tw/handle/123456789/160025
標題: | Functional Analysis of the Subunits of the Chromatin Assembly Factor Rsf | 作者: | Loyola, A. Huang, J.-Y. LeRoy, G. Hu, S. Wang, Y.-H. Donnelly, R. J. Lane, W. S. Lee, S.-C. Reinberg, D. 呂勝春 |
公開日期: | 2003 | 卷: | v.23 | 期: | n.19 | 起(迄)頁: | 6759-6768 | 來源出版物: | MOLECULAR AND CELLULAR BIOLOGY | 摘要: | The human ISWI-containing factor RSF (for remodeling and spacing factor) is composed of two subunits: the ATPase hSNF 2H and p325 (Rsf-1), a protein encoded by a novel human gene. We previously showed that RSF mediates nucleosome deposition and generates regularly spaced nucleosome arrays. Here we report the characterization of the largest subunit of RSF, Rsf-1. We found that Rsf-1 is a highly acidic protein containing a plant homology domain. The present study includes the cloning of Rsf-1, the preparation of recombinant RSF, and the dissection of the role of each subunit in the chromatin assembly reaction. The sequence of the gene for Rsf-1 includes a recently characterized cDNA, HBXAP; postulated to be involved in the transcriptional regulation of the hepatitis B virus. HBXAP actually contains a 252-amino-acid truncation of the amino terminus of Rsf-1. Finally, comparison of HBXAP and Rsf-1 properties shows that they are functionally different. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/92451 |
顯示於: | 分子醫學研究所 |
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