https://scholars.lib.ntu.edu.tw/handle/123456789/160027
標題: | Anti-Ssb/La Is One of the Antineutrophil Autoantibodies Responsible for Neutropenia and Functional Impairment of Polymorphonuclear Neutrophils in Patients with Systemic Lupus Erythematosus | 作者: | HSIEH, S.-C. YU, H.-S. LIN, W.-W. SUN, K.-H. TSAI, C.-Y. HUANG, D.-F. TSAI, Y.-Y. YU, C.-L. 余家利 |
公開日期: | 2003 | 卷: | v.131 | 期: | n.3 | 起(迄)頁: | 506-516 | 來源出版物: | CLINICAL AND EXPERIMENTAL IMMUNOLOGY | 摘要: | Decreased number and impaired functions of polymorphonuclear neutrophils (PMN) due to the presence of anti-PMN autoantibodies in the serum render patients with systemic lupus erythematosus (SLE) susceptible to bacterial infections. However, the cognate antigens and pathological mechanisms of anti-PMN autoantibodies in SLE are rarely reported in the literature. In this study, we found approximately 20% of SLE sera contained anti-PMN autoantibodies detected by human PMN-coated cellular ELISA. A membrane protein with molecular weight of 50 kDa was identified as the cognate antigen of anti-PMN in Western blot after membrane-biotinylation and streptavidin column elution. The 50 kDa molecule was proved to be SSB/La after immunoscreening, molecular cloning and nucleotide sequencing of the gene from the human leucocyte cDNA library. Human anti-SSB/La autoantibodies purified from active SLE sera passing through the recombinant SSB/La conjugated Sepharose 4B affinity column could bind and penetrate into normal human PMN. Functional analysis revealed that the anti-SSB/La autoantibodies exerted a number of potent effects on human PMN , including suppressed phagocytosis, accelerated apoptosis and enhanced IL -8 production. These in vitro results suggest that anti-SSB/La is one of the anti-PMN autoantibodies capable of penetrating into PMN and responsible for neutropenia and functional impairment of PMN in patients with SLE. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/92453 |
顯示於: | 分子醫學研究所 |
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