|Title:||Polyclonal Anticardiolipin Antibodies Purified from Sera of Patients with Active Systemic Lupus Erythematosus Induce Apoptosis of the Cultured Glomerular Mesangial Cells||Authors:||TSAI, CHANG-YU
|Keywords:||anticardiolipin antibodies;apoptosis;glomerular mesangial cell;lupus nephritis;ANTI-DNA ANTIBODIES;ANTIPHOSPHOLIPID ANTIBODIES||Issue Date:||2000||Journal Volume:||v.29||Journal Issue:||n.6||Start page/Pages:||370-379||Source:||SCANDINAVIAN JOURNAL OF RHEUMATOLOGY||Abstract:||
Objective: To test the effect of anticardiolipin antibodies( aCL) on cultured glomerular mesangial cells with regard to their expression of apoptosis-related genes. Methods: aCL purified from active lupus sera by cardiolipin micelles were incubated with cultured rodent mesangial cells ( RMC). Morphological changes of the RMC were observed. The genomic DNA was extracted for the detection of apoptosis. The total cell RNA was extracted for detection of Fas, c-myc, p53, and bcl-2 transcripts by reverse transcription-polymerase chain reaction. Results: aCL (100 GPL-U/0.1 mg protein/ml) bound to RMC more prominent than human IgG (100 mug/ml). The antibodies suppressed RMC proliferation in a dose-dependent manner. The RMC were undergoing apoptosis as evidenced by morphologic changes, fluorescein-annexin V staining and appearance of nucleosome-sized DNA fragments. RMC spontaneously express p53 and c-myc but not Fas or bcl-2. aCL (100 GPL-U/ml) enhanced the expression of Fas but not other apoptosis- related genes and suppressed the intracellular tyrosine phosphorylation. Conclusions: Binding of aCL can induce apoptosis of the RMC. The aCL may be implicated in the pathogenesis of lupus nephritis.
|Appears in Collections:||分子醫學研究所|
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