https://scholars.lib.ntu.edu.tw/handle/123456789/160069
標題: | Functional Interaction between Nuclear Matrix-Associated Hbxap and Nf- Kappa B | 作者: | HUANG, JING-YI SHEN, BIN-JON TSAI, WEN-HAI LEE, SHENG-CHUNG |
關鍵字: | HBXAP;NF-kappa B;nuclear matrix;p50;p65;PHD | 公開日期: | 2004 | 卷: | v.298 | 期: | n.1 | 起(迄)頁: | 133-143 | 來源出版物: | EXPERIMENTAL CELL RESEARCH | 摘要: | Hepatitis B virus X-associated protein (HBXAP) is a plant homeodomain ( PHD) finger-containing protein implicated in transcription regulation. However, the underlying molecular mechanism remains to be defined. Here, we show that HBXAP represses NF-kappaB-mediated gene activation in a dose- dependent manner. Our results showed that HBXAP and NF- kappaB colocalize to the nuclear matrix with specific physical interaction between them. HBXAP may depend on its nuclear matrix localization for its repression of NF-kappaB- mediated gene repression. A specific nuclear matrix targeting sequence of HBXAP was identified. The sequence is included in a region encompassing amino acids 688-722 that could form a coiled-coil structure. The 18-amino acid stretch lies at the core of that structure. The present results showed that either the coiled-coil conformation or the PHD finger domain is crucial for the transcription repression activity of HBXAP on NF -kappaB-mediated gene activation. Taken together, our results suggest that HBXAP may function as a negative regulator for TNF-alpha-induced, NF- kappaB-mediated gene activation. (C) 2004 Elsevier Inc. All rights reserved. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/95089 |
顯示於: | 分子醫學研究所 |
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