Repository logo
  • English
  • 中文
Log In
Have you forgotten your password?
  1. Home
  2. College of Medicine / 醫學院
  3. Molecular Medicine / 分子醫學研究所
  4. Identification and Characterization of ARL4D Interacting Protein VPS4A and Establishment and Phenotypic Function Study of ARL4D Knockout Mice
 
  • Details

Identification and Characterization of ARL4D Interacting Protein VPS4A and Establishment and Phenotypic Function Study of ARL4D Knockout Mice

Date Issued
2009
Date
2009
Author(s)
Chen, Wu-Shan
URI
http://ntur.lib.ntu.edu.tw//handle/246246/178722
Abstract
ARL 4D is a regulated developmentally protein which belongs to ADP-ribosylation factor (ARF) small GTP binding protein family. In previous researches, our lab observed the heterogeneous distributions of constitutively inactive form of ARL4D (ARL4DT35N) at endosomes, mitochondria, and aggresome-like compartments in COS-7 cells. Indeed, ARL4DT35N overexpression diminished mitochondria membrane potential. To characterize function of ARL4DT35N, yeast two-hybrid screening was performed to identify the interacting partner in the mouse embryonic 7-day library.n interacting protein vacuolar protein sorting factor 4A (VPS4A) was chosen for further study. VPS4A (a.a. 286-437) interacts with ARL4DT35N specifically and directly in a GDP-dependent manner. ARL4DT35N and VPS4A co-overexpression in HeLa cells could enhance the cytokinesis inhibition induced by VPS4A. This increase was similar to dominant-negative VPS4AK173Q overexpression. In addition, ARL4D knockdown by siRNA would reduce cytokinesis inhibition induced by VPS4AK173Q. Together, these data suggested ARL4DT35N might affect the function of VPS4A, and subsequently affect the cytokinesis.RL4D knockout mice have been generated to investigate the physiological function of ARL4D. ARL4D knockout mice were viable and fertile. However, there were many vacuoles in the liver section of male ARL4D knockout mice by hematoxylin and eosin staining, representing primary degeneration of liver function; severe inflammation was also observed in liver of a female ARL4D knockout mouse. Data of blood chemistry analysis indicated the enzyme activities of GOT, GPT, and CPK were higher in ARL4D knockout mice than wild-type and heterozygous mice. This increase was significant in male mice. More samples need to be examined before giving the final conclusion.
Subjects
ARL4D
ARF
yeast twouhybrid screening
cytokinesis
knockout mice
File(s)
Loading...
Thumbnail Image
Name

ntu-98-R95448005-1.pdf

Size

23.32 KB

Format

Adobe PDF

Checksum

(MD5):e943c0b2182d69a5c449a39342c4bd52

臺大位居世界頂尖大學之列,為永久珍藏及向國際展現本校豐碩的研究成果及學術能量,圖書館整合機構典藏(NTUR)與學術庫(AH)不同功能平台,成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。

To permanently archive and promote researcher profiles and scholarly works, Library integrates the services of “NTU Repository” with “Academic Hub” to form NTU Scholars.

總館學科館員 (Main Library)
醫學圖書館學科館員 (Medical Library)
社會科學院辜振甫紀念圖書館學科館員 (Social Sciences Library)

開放取用是從使用者角度提升資訊取用性的社會運動,應用在學術研究上是透過將研究著作公開供使用者自由取閱,以促進學術傳播及因應期刊訂購費用逐年攀升。同時可加速研究發展、提升研究影響力,NTU Scholars即為本校的開放取用典藏(OA Archive)平台。(點選深入了解OA)

  • 請確認所上傳的全文是原創的內容,若該文件包含部分內容的版權非匯入者所有,或由第三方贊助與合作完成,請確認該版權所有者及第三方同意提供此授權。
    Please represent that the submission is your original work, and that you have the right to grant the rights to upload.
  • 若欲上傳已出版的全文電子檔,可使用Open policy finder網站查詢,以確認出版單位之版權政策。
    Please use Open policy finder to find a summary of permissions that are normally given as part of each publisher's copyright transfer agreement.
  • 網站簡介 (Quickstart Guide)
  • 使用手冊 (Instruction Manual)
  • 線上預約服務 (Booking Service)
  • 方案一:臺灣大學計算機中心帳號登入
    (With C&INC Email Account)
  • 方案二:ORCID帳號登入 (With ORCID)
  • 方案一:定期更新ORCID者,以ID匯入 (Search for identifier (ORCID))
  • 方案二:自行建檔 (Default mode Submission)
  • 方案三:學科館員協助匯入 (Email worklist to subject librarians)

Built with DSpace-CRIS software - Extension maintained and optimized by 4Science