Functional characterization of Ser-428-Pro and RRMs mutations in Rbp1p
Date Issued
2009
Date
2009
Author(s)
Liu, Yi-Yun
Abstract
RNA-binding proteins forming dynamic messenger ribonucleoproteins with the transcript are required for the regulation of eukaryotic gene expression. The RNA-binding protein RBP1 encodes a 672-amino acid, ~80-kD protein containing three RNA recognition motifs (RRM) and two glutamine-rich stretches. It is first identified as a negative growth regulator. Our lab has demonstrated overexpression of Rbp1p shows a slow-growth phenotype, decreases the porin mRNA level by enhancing degradation, and suppresses the translation. Rbp1p localized to cytoplasm foci, named P-bodies. Here we showed that serine-428-Proline mutation loses the function of Rbp1p in slow-growth, translation repression and decreasing the stability of POR1 mRNA, but it still can localize to P-bodies. Rbp1p-S428P and Rbp1p RRM motif mutants induce flocculation and invasive growth. Flocculins, FLO1 and FLO11, are membrane surface glycoproteins for adhesion. FLO1 deletion suppresses flocculation; however FLO11 deletion decreases the invasive-growth level. It is also demonstrated that MSS11, transcription factor of FLO1 and FLO11 is required for both adhesion phenotypes, suggesting RNA-binding proteins mutation might regulate flocculation and invasive growth through MSS11. Rbp1p interacting proteins, DHH1, XRN1 and KRE6 might be possible candidates interacting with Rbp1p-S428P and Rbp1p RRM motif mutants to regulate adhesion phenotypes.
Subjects
RNA-binding proteins
RNA recognition motifs
flocculation
invasive growth
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