https://scholars.lib.ntu.edu.tw/handle/123456789/160291
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor | 臺大醫學院-分子醫學研究所; | - |
dc.contributor.author | Huang, Lien-Hung | en |
dc.contributor.author | Lee, Wei-Chung | en |
dc.contributor.author | You, Shu-Ting | en |
dc.contributor.author | Cheng, Chia-Chen | en |
dc.contributor.author | Yu, Chia-Jung | en |
dc.contributor.author | Wanjin, Hong | en |
dc.creator | Huang, Lien-Hung; Lee, Wei-Chung; You, Shu-Ting; Cheng, Chia-Chen; Yu, Chia-Jung; Wanjin, Hong | en |
dc.date | 2015 | - |
dc.date.accessioned | 2017-05-25T07:31:30Z | - |
dc.date.accessioned | 2018-07-09T01:23:59Z | - |
dc.date.available | 2017-05-25T07:31:30Z | - |
dc.date.available | 2018-07-09T01:23:59Z | - |
dc.date.issued | 2015 | - |
dc.identifier.uri | http://ntur.lib.ntu.edu.tw//handle/246246/278988 | - |
dc.description.abstract | The small GTPase Arf-like protein 1 (Arl1) is well known for its role in intracellular vesicular transport at the trans-Golgi network (TGN). In this study, we used differential affinity chromatography combined with mass spectrometry to identify Arf-interacting protein 1b (arfaptin- 1b) as an Arl1-interacting protein and characterized a novel function for arfaptin-1 (including the arfaptin-1a and 1b isoforms) in Arl1-mediated retrograde transport. Using a Shiga-toxin subunit B (STxB) transportation assay, we demonstrated that knockdown of arfaptin-1 accelerated the retrograde transport of STxB from the endosome to the Golgi apparatus, whereas Arl1 knockdown inhibited STxB transport compared with control cells. Arfaptin-1 overexpression, but not an Arl1 binding-defective mutant (arfaptin-1b-F317A), consistently inhibited STxB transport. Exogenous arfaptin-1 expression did not interfere with the localization of the Arl1-interacting proteins golgin-97 and golgin-245 to the TGN and vice versa. Moreover, we found that the N-terminal region of arfaptin-1 was involved in the regulation of retrograde transport. Our results show that arfaptin-1 acts as a negative regulator in Arl1-mediated retrograde transport and suggest that different functional complexes containing Arl1 form in distinct microdomains and are responsible for different functions. | - |
dc.language | en-us | - |
dc.relation | PLoS One, 10(3), | - |
dc.relation.ispartof | PLoS ONE | en_US |
dc.title | Arfaptin-1 Negatively Regulates Arl1-Mediated Retrograde Transport | - |
dc.identifier.doi | 10.1371/journal.pone.0118743 | - |
dc.relation.pages | e0118743 | - |
item.fulltext | no fulltext | - |
item.grantfulltext | none | - |
顯示於: | 分子醫學研究所 |
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