The role of PTPN22 in regulating c-Maf activity
Date Issued
2007
Date
2007
Author(s)
Yen, Wei-Feng
DOI
en-US
Abstract
The transcription factor c-Maf plays different roles in different types of cells. For instance, c-Maf is the IL-4–specific transcription factor in TH2 cells. Besides, c-Maf is a potent activator of the IL-10 gene expression whereas c-Maf could suppress the IL-12 p40 and p35 gene transcription in macrophages. We hypothesize that c-Maf could have distinct functions through interacting with cell-specific protein in different types of cells. We screened the c-Maf interacting protein in CD8+ T cell library by yeast two-hybrid system. Fifteen candidate genes were obtained. One of the candidate genes encoded a protein-tyrosine phosphatase, PTPN22. Here, we demonstrated that PTPN22 can express in primary resting or activated CD4+ T helper cells and c-Maf can interact with PTPN22 in vitro. Furthermore, we demonstrated that the PTPN22 had the ability to diminish the transactivity of c-Maf on IL-4 promoter. We also found that the tyrosine residues of c-Maf could be phosphorylated in HEK 293T cell line. Moreover, we found that ectopic-expressed DsRed2-c-Maf and EYFP-PTPN22 could co-localize in the nucleus. However, the functions of phosphorylated c-Maf in vivo need to be further elucidate.
Subjects
細胞激素
輔助型T細胞
c-Maf
Type
other