Repository logo
  • English
  • 中文
Log In
Have you forgotten your password?
  1. Home
  2. College of Medicine / 醫學院
  3. Immunology / 免疫學研究所
  4. Role of co-stimulatory molecules and IL-4 in the generation of long-term CD4+ T cell memory
 
  • Details

Role of co-stimulatory molecules and IL-4 in the generation of long-term CD4+ T cell memory

Date Issued
2005
Date
2005
Author(s)
Yeh, Tzu-Lin
DOI
en-US
URI
http://ntur.lib.ntu.edu.tw//handle/246246/63342
Abstract
記憶型T淋巴球可抵抗對外來抗原,在免疫系統當中扮演重要的角色。 然而,對於控制記憶型CD4+ T淋巴球產生的詳細機制卻還不甚清楚。 在本研究當中,我們嘗試著去研究輔助刺激因子(costimulatory molecule)是否參與在記憶型CD4+ T淋巴球的生成。 經由cDNA的轉染(transfection)製造出表現在細胞表面帶有CD24、CD40、CD70、CD86、CD153、OX40L、4-1BBL和IcosL的EL4腫瘤細胞株。 另外,為了使這些轉染細胞株(transfectant)可以當作抗原呈現細胞(antigen-presenting cell)以活化CD4+ T淋巴球,因此在每種轉染細胞株的細胞表面上再加以轉染FcgRI,使轉染細胞株可以結合上anti-CD3單株抗體,並將之呈現給CD4+ T淋巴球以刺激之。 在活體外實驗的結果發現,除了CD28:CD86,在其他輔助型因子的刺激之下,CD4+ T淋巴球的活化狀況都不是很好。 因此在利用轉染細胞株EL4活化CD4+ T淋巴球時,我們同時加入anti-CD3和anti-CD28的刺激。 CD4+ T淋巴球在帶有各種不同輔助型刺激因子轉染細胞株EL4的刺激之後,再打入宿主老鼠體內,我們發現細胞表面表現CD40的轉染細胞株,可提供CD40:CD40L的刺激,造成大量長期存活的CD4+ T淋巴球。 另外,我們還發現CD4+ T淋巴球在B淋巴球的刺激之下再外加第四介白素也可以幫助生成長期存活的CD4+ T淋巴球。 至於其他的輔助刺激因子,包括CD28:CD86、CD27:CD70、CD30:CD153、OX40:OX40L、Icos:IcosL和4-1BB:4-1BBL,則對於記憶型CD4 T 淋巴球的生成沒有正向的影響。以上的研究發現對於腫瘤的治療與疫苗的開發皆有所幫助。
Long-lived memory T cells play important roles in the defense against foreign invaders, although the mechanisms involved in memory T cell generation are not fully understood. In this study, I studied the effects costimulatory molecules play on memory CD4+ T cell memory generation. EL4 tumor cell lines that had through transfection been made to stably co-express FcgRI and one or combinations of CD24, CD40, CD70, CD86, CD153, OX40L, 4-1BBL, and IcosL costimulators were used as antigen-presenting cells to activate CD4+ T cells. Such transfectants delivered signal 1 through anti-CD3 mAbs presented by its FcgRI and signal 2 through the given costimulatory molecules it expresses. Except CD28:CD86, all other costimulatory molecules were ineffective at causing CD4+ T cell proliferation. To insure proper activation of CD4 T cells, anti-CD3 and anti-CD28 were both added and were presented via the FcgRI on the various costimulator-expressing EL4 transfectants. Activated CD4+ T cells were adoptively transferred into histocompatible, congenic hosts and their presence was monitored over time. CD4+ T cells activated by anti-CD3/CD28 in the context of CD40:CD154 developed long-term survival potential. In addition, CD4+ T cells activated by anti-CD3/CD28 in the context of B cells + IL-4 also developed long-term survival potential. All other costimulators, CD28:CD86, CD27:CD70, CD30:CD153, OX40:OX40L, Icos:IcosL, and 4-1BB:4-1BBL, did not have positive effects on long-term CD4 memory generation. These results may have implications in tumor therapy and vaccine development.
Subjects
輔助刺激因子
第四介白素
記憶型 CD4+ T 淋巴球
co-stimulatory molecules
IL-4
memory CD4 T cell
SDGs

[SDGs]SDG3

Type
other
File(s)
Loading...
Thumbnail Image
Name

ntu-94-R92449004-1.pdf

Size

23.31 KB

Format

Adobe PDF

Checksum

(MD5):aeaa0084341091d5d90dfef7b107a3cb

臺大位居世界頂尖大學之列,為永久珍藏及向國際展現本校豐碩的研究成果及學術能量,圖書館整合機構典藏(NTUR)與學術庫(AH)不同功能平台,成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。

To permanently archive and promote researcher profiles and scholarly works, Library integrates the services of “NTU Repository” with “Academic Hub” to form NTU Scholars.

總館學科館員 (Main Library)
醫學圖書館學科館員 (Medical Library)
社會科學院辜振甫紀念圖書館學科館員 (Social Sciences Library)

開放取用是從使用者角度提升資訊取用性的社會運動,應用在學術研究上是透過將研究著作公開供使用者自由取閱,以促進學術傳播及因應期刊訂購費用逐年攀升。同時可加速研究發展、提升研究影響力,NTU Scholars即為本校的開放取用典藏(OA Archive)平台。(點選深入了解OA)

  • 請確認所上傳的全文是原創的內容,若該文件包含部分內容的版權非匯入者所有,或由第三方贊助與合作完成,請確認該版權所有者及第三方同意提供此授權。
    Please represent that the submission is your original work, and that you have the right to grant the rights to upload.
  • 若欲上傳已出版的全文電子檔,可使用Open policy finder網站查詢,以確認出版單位之版權政策。
    Please use Open policy finder to find a summary of permissions that are normally given as part of each publisher's copyright transfer agreement.
  • 網站簡介 (Quickstart Guide)
  • 使用手冊 (Instruction Manual)
  • 線上預約服務 (Booking Service)
  • 方案一:臺灣大學計算機中心帳號登入
    (With C&INC Email Account)
  • 方案二:ORCID帳號登入 (With ORCID)
  • 方案一:定期更新ORCID者,以ID匯入 (Search for identifier (ORCID))
  • 方案二:自行建檔 (Default mode Submission)
  • 方案三:學科館員協助匯入 (Email worklist to subject librarians)

Built with DSpace-CRIS software - Extension maintained and optimized by 4Science