Regulation of murine IL-21 gene by c-Maf
Date Issued
2009
Date
2009
Author(s)
Yang, Fu-Wei
Abstract
c-Maf is a b-Zip transcription factor, which was found to be an essential role in regulating IL-4 expression in Th2 cells. Recent study has investigated the relationship between c-Maf and IL-21 gene expression in Th17 cells. Their study uncovered shortage of IL-21 production in c-Maf KO mice, which implied that c-Maf might be an upstream molecule of IL-21. Since c-Maf itself is a transcription factor, we hypothesized that c-Maf should regulate IL-21 gene expression. To identify whether c-Maf regulates IL-21 expression by directly or indirectly binding on IL-21 promoter, we went to investigate MARE sequence on IL-21 promoter. We found two half MARE and two V-MARE in -1.3kb upstream region. We constructed several point mutatated and truncated promoter fragments onto pGL3 Basic vector, and performed luciferase assay. Our results pointed out that the putative c-Maf binding site should be V-MARE locating -192 ~ -205kb. On the other hand, the SUMOylation effect of c-Maf on IL-21 gene expression was tested. We concluded that c-Maf transactivation ability on IL-21 gene expression was not affected by SUMOylation.
Subjects
c-Maf
IL-21
Th17
File(s)![Thumbnail Image]()
Loading...
Name
ntu-98-R96449002-1.pdf
Size
23.32 KB
Format
Adobe PDF
Checksum
(MD5):3eb5743bf649d5f55675fab967af9d67