Characterization of Runx3-modulated IFN-γ expression
Date Issued
2010
Date
2010
Author(s)
Cho, Yi-Li
Abstract
Runx proteins, which contain a conserved Runt domain, are a heterodimer bound with cofactor, Cbfβ. Runx family contains three members, Runx1, Runx2, and Runx3. Runx3, the smallest protein of the family, mainly expressed in effector CD4+ Th1 cells and CD8+ T cells and cooperates with T-bet to activate IFN-γ gene expression. However, which domain(s) of Runx3 interacts/interact with T-bet to drive IFN-γ expression remains unknown. P054 mice, generated from ENU core in Academia Sinica, have a truncated Runx3 protein with 105 amino acid deletion in C-terminal. We observed abnormal profile of CD4/CD8 expression in P054 mice. Furthermore, decreased IFN-γ productions were identified in Th1 and CD8 of P054 mice. It indicates that the deleted C-terminal domain of Runx3 is important in T cell development and their effecor functions. However, loss of Runx3 C-terminal still maintains its cellular localization and protein stability. In addition, we observed that the NMTS domain of Runx3 is an important domain to cooperate with T-bet for the activation of IFN-γ. Take together, The NMTS domain of Runx3 might play a important role in lymphoid cell development and IFN-γ production.
Subjects
Runx3
IFN-γ
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-99-R97449006-1.pdf
Size
23.32 KB
Format
Adobe PDF
Checksum
(MD5):9359bc9d72183f1658e3671d97c25ce8