DC 欄位 | 值 | 語言 |
dc.contributor.author | 許世明 | zh_TW |
dc.creator | 許世明 | zh_TW |
dc.date | 2004 | zh_TW |
dc.date.accessioned | 2018-07-09T01:47:35Z | - |
dc.date.available | 2018-07-09T01:47:35Z | - |
dc.date.issued | 2004 | - |
dc.identifier | 922314B002118 | zh_TW |
dc.identifier.uri | http://ntur.lib.ntu.edu.tw//handle/246246/25363 | - |
dc.description.abstract | 人體周邊血液中有二種主要的自然殺手細胞,分別有顯著不同的表面抗原,
CD56 + KIR +/- Lectin + 及CD 56 ± KIR + Lectin ± ,本計劃將重細胞發生學的觀點來
澄清這兩種自然殺手細胞的來源。
本計劃至目前為止,已完成下列四項主要目標:
1. 臍帶血CD34 + 幹細胞之分離及擴增
我們已成功將CD34 + 幹細胞由臍帶血分離,在混合白血球介質刺激下,經
培養1~2 個星期後,可以擴增5~10 倍。
2. CD56 表現於約10%的CD34 - 細胞
經過另一組白血球的介質的刺激,我們可以於10%的CD34 - 細胞中發現有
CD56 的表現,這CD56 + 細胞最有可能是NK 母細胞,但是骨髓胜原始細胞
亦不可能排除,我們將進一步分析之。
3. 鼻咽淋巴癌之系源分析
我們用自然殺手細胞類免疫球蛋白之限制性分布,作有診斷及系源認定的標
準,我們發現,大部份鼻咽淋巴癌源自於自然殺手細胞(Am J Path 2001. 159.
1671 )
4.鼻咽淋巴癌之臨床分析
我們更進一步以白血球表面抗原94 (CD94 )分析其鼻咽淋巴癌之臨床表現,
發現鼻咽淋巴癌分為CD94 + 及CD94 - 兩種亞型, CD94 + 明顯有較佳預後
(Blood 2003. 102. 2623 )我們目前正進行比對,以確定這2 種亞型是否就是
分別由CD56 + KIR +/- Lectin + 及CD 56 ± KIR + Lectin ± 這兩種自然殺手細胞演變而
來。 | zh_TW |
dc.description.abstract | There are 2 major subtypes of mature NK-cells with differential surface markers in the
peripheral blood, CD56 + KIR +/- Lectin + and CD56 +/- KIR + Lectin +/- . Our proposal is aimed at
clarifying the developmental pathway of these 2 subtypes of mature NK-cells. So far, we have
achieved the following:
I: Isolation and expansion of CD34 + stem cells from umbilical cord blood
We have isolated CD34 + stem cells from umbilical cord. We are able to expand the CD34 + Lin-
stem cells with the use of an in vitro culture system under cytokine stimulation. An 5-10 fold
expansion is achieved after 1-week culture.
II: Partial Induction of CD56 expression, suggestive of NK-differentiation
The expanded stem cells were further grown for another 1 week, to induce differentiation.
Although most cells developed into the myeloid lineage, about 5-10% were CD56 + , suggestive
of an NK-lineage. To continue the investigation, we are currently using IL-15 to stimulate
proliferation and induction of mature NK-cells.
III: Confirmation of lineage assignment of sinonasal lymphoma
We have performed an extensive assessment of the lineage assignment of sinonasal
lymphomas. We found most sinonasal lymphomas had a restricted killer immunoglobulin-like
receptor repertoire, consistent with a NK-lineage. But some cases had a monoclonal T-cell
receptor rearrangement plus a restricted killer immunoglobulin-like receptor repertoire, consistent
with a mixed NK/T cell lineage. (Am J Path, 2001, 159, 1671.)
IV: Clinical correlation and significance.
Further analysis of the expression pattern of CD94 (the killer lectin-like receptor) showed
that a subset of sinonasal lymphoma had CD94 expression. The CD94 positive subset had a much
better prognosis than the CD94-negative subset. (Blood, 2003, 102, 2623) We are currently
investigating whether these 2 subsets correspond to the 2 major subsets of mature NK-cells
of the peripheral blood. | en |
dc.format | application/pdf | zh_TW |
dc.format.extent | 76595 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language | zh-TW | zh_TW |
dc.language.iso | zh_TW | - |
dc.publisher | 臺北市:國立臺灣大學醫學院免疫學研究所 | zh_TW |
dc.rights | 國立臺灣大學醫學院免疫學研究所 | zh_TW |
dc.subject | 雙系模式 | zh_TW |
dc.subject | 細胞介質15(IL15) | zh_TW |
dc.subject | 細胞介質21(IL21) | zh_TW |
dc.subject | 白血球
表面抗原56(CD56) | zh_TW |
dc.subject | 自然殺手細胞類免疫球蛋白(KIR) | zh_TW |
dc.subject | 糖
類接合蛋白(Lectin) | zh_TW |
dc.subject | 白血球球表面抗原94(CD94) | zh_TW |
dc.subject | dual lineage | en |
dc.subject | IL15 | en |
dc.subject | IL21 | en |
dc.subject | CD56 | en |
dc.subject | KIR | en |
dc.subject | lectin | en |
dc.title | 自然弒細胞發生學的雙系模式:白血球介質15及21分別導引 CD56+Kir±lectis+ 及 CD56±Kirlectin自然弒細胞(2/3) | zh_TW |
dc.title | A dual lineage model for the development of mature human NK-cells(2/3) | en |
dc.type | journal article | en |
dc.identifier.uri.fulltext | http://ntur.lib.ntu.edu.tw/bitstream/246246/25363/1/922314B002118.pdf | - |
dc.coverage | 計畫年度:92;起迄日期:2003-08-01/2004-07-31 | zh_TW |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.languageiso639-1 | zh_TW | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.fulltext | with fulltext | - |
顯示於: | 免疫學研究所
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