https://scholars.lib.ntu.edu.tw/handle/123456789/160559
標題: | Both Cxcr3 and Cxcl10/Ifn-Inducible Protein 10 Are Required for Resistance to Primary Infection by Dengue Virus | 作者: | SUNG, JUI-MIN WU-HSIEH, BETTY 伍安怡 |
關鍵字: | CENTRAL-NERVOUS-SYSTEM;CD8(+) T-CELLS;GAMMA-INDUCIBLE PROTEIN-10;CHEMOKINE EXPRESSION;IN-VIVO;HOST-DEFENSE | 公開日期: | 2006 | 卷: | v.177 | 期: | n.3 | 起(迄)頁: | 1855-1863 | 來源出版物: | JOURNAL OF IMMUNOLOGY | 摘要: | We examined the extent to which CXCR3 mediates resistance to dengue infection. Following intracerebral infection with dengue virus, CXCR3- deficient (CXCR3(-/-)) mice showed significantly higher mortality rates than wild-type (WT) mice; moreover, surviving CXCR3(-/-) mice, but not WT mice, often developed severe hind-limb paralysis. The brains of CXCR3-/- mice showed higher viral loads than those of WT mice, and quantitative analysis using real-time PCR, flow cytometry, and immunohistochemistry revealed fewer T cells, CD8(+) T cells in particular, in the brains of CXCR3(-/)- mice. This suggests that recruitment of effector T cells to sites of dengue infection was diminished in CXCR3-/- mice, which impaired elimination of the virus from the brain and thus increased the likelihood of paralysis and/or death. These results indicate that CXCR3 plays a protective rather than an immunopathological role in dengue virus infection. In studies to identify critical CXCR3 ligands, CXCL10/IFN- inducible protein 10-deficient (CXCL10/IP-10(-/-)) mice infected with dengue virus showed a higher mortality rate than that of the CXCR3(-/-) mice. Although CXCL10/IP-10, CXCL9/monokine induced by IFN-gamma, and CXCL 11/IFN- inducible T cell a chemoattractant share a single receptor and all three of these chemokines are induced by dengue virus infection, the latter two could not compensate for, the absence of CXCL10/IP-10 in this in vivo model. Our results suggest that both CXCR3 and CXCLIO/IP-10 contribute to resistance against primary dengue virus infection and that chemokines that are indistinguishable in in vitro assays differ in their activities in vivo. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/94216 |
顯示於: | 免疫學研究所 |
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