Study on the role of Galectins in the regulatory function of Treg-of-B2 cells
Date Issued
2014
Date
2014
Author(s)
Yang, Chih-Chieh
Abstract
Regulatory T cells (Treg cells) have an ability to suppress immune response. Under particular condition, naive CD4+ T cell could differentiate into inducible Treg (iTreg) cells with regulatory functions. In the past few years, some in vitro experiments have shown that splenic B220+ B2 cells could convert naive CD4+ T cells into induced Treg cells (referred to as Treg-of-B2 cells). Treg-of-B2 cells had immune suppressive ability like nTreg cells, but the mechanism remained unclear. To date, 15 galectins have been identified in mammals, only galectin-1, -2, -3, -4, -7, -8, -9, -12 identified in mice. Recent studies have shown that several galectins (including galectin-1, -2, -3, -7, -8, -9, and -12) induced apoptosis in T cell. Galectin-1 was a key effector molecule in the regulation function of CD4+CD25+ T cells. Galectin-2 induced activated CD8+ T cells apoptosis. Galectin-3 exerted several functions such as inhibition of apoptosis, promotion of cell growth, and regulation of TCR signaling. The aim of our study was to investigate the relationship between galectins and the regulatory function of Treg-of-B cells. First, expression profile of galectins in activated Treg-of-B2 cells and activated Tr1 cells had been screened. We found that activated Treg-of-B2 cells had higher galectin-2 expression but Tr1 cells did not. Second, the suppressive function of Treg-of-B2 cells did not need cell-cell contact. By adding lactose and transwell system in suppressive assay, we found that galectin-2 might be a factor in the function of Treg-of-B2 cells. We confirmed the role of galectin-2 in the proliferation of CD4+ CD25- T cells by adding recombinant galectin-2 in culture medium and we found that the proliferation of CD4+ CD25- T cells was decreased. Our finding clarify that a part of suppressive ability of Treg-of-B2 cells was galectin-2 secretion.
Subjects
半乳糖凝集素-2
調節性T細胞
Treg-of-B2細胞
Type
thesis
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