|Title:||Swept Source Optical Coherence Tomography for Radiation-Enhanced Hepatocellular Carcinoma Cell Invasion Imaging||Authors:||KUO, WEN-CHUAN
CHENG, JASON CHIA-HSIEN
|Issue Date:||2009||Start page/Pages:||4289-4297||Source:||Physics in Medicine and Biology||Abstract:||
In this study, we evaluated the effectiveness of using swept -source optical coherence tomography (SS-OCT) to monitor the enhancement of hepatocellular carcinoma (HCC) cell invasiveness by radiation. SS-OCT images were acquired and recorded to obtain three-dimensional datasets at discrete time points of 12, 24 and 48 h after irradiating HepG2 cells with 7.5 Gy. The cell migration distance in three- dimensional tissue models was quantified from images of radiation-induced and sham-irradiated cells, and this method was compared with the conventional Boyden chamber assay conducted at the same time points. SS-OCT measurements show that most cells were found near the gel surface, but a few were much deeper. Among those HCC cells with a high degree of migration capability, the mean migration distances at 24 h and 48 h were significantly greater for irradiated cells than for sham-irradiated cells (0.7 +/- 0.23 mm versus 0. 65+ /- 0.26 mm at 24 h, P = 0.019 and 0.84 +/- 0.30 mm versus 0. 65 +/- 0. 524 mm at 48 h, P = 0.009). The results of radiation-enhanced invasion in HCC cells obtained by the non -invasive, quantitative SS-OCT method were consistent with those obtained using the traditional assay for measuring biological invasion.
|Appears in Collections:||腫瘤醫學研究所|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.