|Title:||Nanocrystalline calcium sulfate/hydroxyapatite biphasic compound as a TGF-beta 1/VEGF reservoir for vital pulp therapy||Authors:||Chiang, Yu-Chih
|Keywords:||Nanocrystalline calcium sulfate;TGF-beta 1;VEGF;Micro-computed tomography;Vital pulp therapy||Issue Date:||2016||Journal Volume:||32||Journal Issue:||10||Start page/Pages:||1197-1208||Source:||Dent. Mater.||Abstract:||
Objectives. Vital pulp therapy aims to treat reversible pulpal injuries via protective dentinogenesis and to preserve more tooth structure. Mineral trioxide aggregate (MTA)-based capping materials demonstrate prolonged setting time increases the risk of pulpal infection during multi-visit treatment. Their non-degradable property occupies pulp space and limits dentin-pulp regeneration. This study reports an inorganic degradable biomaterial that presents a short initial setting time and acts as a growth factor reservoir to promote reparative dentinogenesis. ;Methods. We synthesize nanocrystalline calcium sulfate hemihydrate (nCS), hydroxyapatite (HAp) and calcium sulfate hemihydrate (CS) as a reservoir to which transforming growth factor-beta 1 (TGF-beta 1) and vascular endothelial growth factor (VEGF) are added (denoted as nCS/HAp/CS/TGF-beta 1NEGF). In vitro biocompatibility and mineralization (the activity and expression of alkaline phosphatase, ALP) were evaluated. Rat animal model was created to test in vivo efficacy. ;Results. Cultured human dental pulp cells (HDPCs) showed that nCS/HAp/CS/TGF-beta 1/VEGF cement has excellent biocompatibility and the potential to elevate the activity and expression of ALP. The in vivo efficacy (rat animal model) indicates protective dentin by micro-computed tomography (mu-CT) measurements and histological analyses. The 3D mu-CT non-destructive analysis also determines volume changes during pulpotomy, suggesting that the degraded space of the nCS/HAp/CS/TGF-beta 1NEGF cement is repaired by the formation of dentin-pulp tissue. ;Significance. These findings demonstrate that nCS/HAp/CS cement acts as a potent reservoir for the sustained release of growth factors, and that nCS/HAp/CS/TGF-beta 1/VEGF cement has a high potential to form the reparative dentinogenesis in vivo. (C) 2016 The Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.
|Appears in Collections:||臨床牙醫學研究所|
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