https://scholars.lib.ntu.edu.tw/handle/123456789/162621
標題: | 在分子層面探討肌肉疼痛之成因:疲乏及正常咀嚼肌中腺核甘三磷酸感受性鉀通道三度空間結構及功能性質之研究 Involvement of a Novel C-Terminal Kinase Domain of Kir6.2 in the K-ATP Channel Rundown Reactivation |
作者: | 樓國隆 Lou, Kuo-Long |
關鍵字: | Channel gating;3-D homology modelling;Kinase domain | 公開日期: | 2000 | 出版社: | 臺北市:國立臺灣大學醫學院口腔生物科學研究所 | 摘要: | Rundown is a generally encountered problem while recording KATP channel activity with inside-out patches. No assigned structural fragment related to this mechanism could be derived from any of the proceeded functional analyses as yet. Therefore, based on a combined sequence and secondary structure alignment against known crystal structure of segments from closely related proteins, we propose here the three-dimensional structure model of an intracellular C-terminal domain of the Kir6.2 subunit in KATP channels. An E. coli CMP-kinase was suggested as template for the model building. The subdomain arrangement of this novel kinase domain and the structural correlation for UDP-docking were described. With structural-functional interpretation, we conclude that the reactivation of KATP channel rundown by MgATP or UDP is very possibly regulated by this intracellular kinase domain at the Cterminus of Kir6.2 subunit in KATP channels. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/22654 | 其他識別: | 892314B002258 | Rights: | 國立臺灣大學醫學院口腔生物科學研究所 |
顯示於: | 口腔生物科學研究所 |
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