https://scholars.lib.ntu.edu.tw/handle/123456789/162622
Title: | 轉醣鏈球菌中各種葡萄糖傳遞酵素之三維結構測定與功能分析 Three-dimensional modelling of the catalytic domain of Streptococcus mutans glucosyltransferase GtfB |
Authors: | 樓國隆 Lou, Kuo-Long |
Keywords: | Glucosyltransferase;Streptococcus mutans;Sucrase activity;Catalytic domain;Three-dimensional model;Tim-barrel | Issue Date: | 2001 | Publisher: | 臺北市:國立臺灣大學醫學院口腔生物科學研究所 | Start page/Pages: | 75-79 | Source: | FEMS Microbiology Letters 2000(188) | Abstract: | Glucosyltransferases (GtfB/C/D) of Streptococcus mutans, a pathogen for human dental caries, synthesize water-insoluble glucan through the hydrolysis of sucrose. Genetic and biochemical approaches have identified several active sites of these enzymes, but no three-dimensional (3D) structural evidence is yet available to elucidate the subdomain arrangement and molecular mechanism of catalysis. Based on a combined sequence and secondary structure alignment against known crystal structures of segments from closely related proteins, we propose here the 3D model of an N-terminal domain essential for the sucrose binding and splitting in GtfB. A Tim-barrel of (K/L)8 structural characteristics is revealed and the structural correlation for two peptides is described. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/22657 | Other Identifiers: | 892314B002555 | Rights: | 國立臺灣大學醫學院口腔生物科學研究所 |
Appears in Collections: | 口腔生物科學研究所 |
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892314B002555.pdf | 601.44 kB | Adobe PDF | View/Open |
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