研究第二型登革病毒血清型專一性抗體的B細胞抗原決定位及評估其為偵檢試劑之可行性
Date Issued
2003
Date
2003
Author(s)
吳漢忠
DOI
912314B002257
Abstract
Serotype-specific monoclonal antibody
(MAb), D2 16-1 (Ab4), against dengue virus
type 2 (DEN-2) was generated. This MAb
recognized non-structural protein 1 (NS1) of
DEN-2; its specificity was determined by
ELISA, immunofluorescence and
immunoblotting analysis. Serotype-specific
B-cell epitope of Ab4 was further identified
from a random phage-displayed peptide
library. Selected phage clones specifically
reacted with Ab4 and did not react with other
MAbs. Immunopositive phage clones
displayed a consensus motif,
His-Arg/Lys-Leu/Ile. Synthetic peptide
corresponding to phage-borne peptide
specifically bound to antibody. His and Arg
in this epitope were crucial for peptide
binding to Ab4: binding activity dramatically
decreased when His or Arg was changed to
Leu. Epitope-based synthetic peptide
identified serum samples from
DEN-2-immunized mice and rabbits by
ELISA assay. The peptide clearly
differentiated between serum samples from
DEN-2- and Japanese encephalitis virus
(JEV)-immunized mice. This monoclonal
antibody and its epitope-based peptide
antigen will be useful for serologic diagnosis
of DEN-2 infection. Furthermore, DEN-2
epitope identification makes it feasible to
dissect the antibody response and to address
the role of antibodies in the pathogenesis of
primary and secondary DEN-2 infections.
Subjects
monoclonal antibody
B-cell
epitope
epitope
synthetic peptide
serologic
diagnosis
diagnosis
SDGs
Publisher
臺北市：國立臺灣大學醫學院口腔生物科學研究所
Type
journal article
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