DC Field | Value | Language |
dc.contributor.author | 吳漢忠 | zh_TW |
dc.creator | 吳漢忠 | zh_TW |
dc.date | 2003 | zh_TW |
dc.date.accessioned | 2006-07-26T03:04:08Z | - |
dc.date.accessioned | 2018-07-09T05:27:13Z | - |
dc.date.available | 2006-07-26T03:04:08Z | - |
dc.date.available | 2018-07-09T05:27:13Z | - |
dc.date.issued | 2003 | - |
dc.identifier | 912314B002257 | zh_TW |
dc.identifier.uri | http://ntur.lib.ntu.edu.tw//handle/246246/22667 | - |
dc.description.abstract | 本計畫主要是要研究登革病毒的B 細
胞抗原決定位。經由ELISA,
immunofluorescence 和immunoblotting 方
法,我們找出具專一性的第二型登革病毒
單株抗體(Ab4)。此抗體之抗原決定位的確
認,有助於病毒感染機制的暸解,疫苗及
偵檢試劑的研發。在此研究中,我們以噬
菌體顯現法來研究第二型登革病毒的抗原
決定位。我們也已經篩選出多株的噬菌體
可以專一性的與第二型登革病毒抗體結
合。這些專一性噬菌體所攜帶的外來
peptide,都含有His-Arg/Lys-Leu/Ile motif。
以synthetic peptide 代替噬菌體所攜帶的外
來peptide,可與第二型登革病毒抗體明顯
反應。第二型登革病毒感染之老鼠及兔子
血清,可以用此epitope-based peptide 來確
認。另外,此epitope-based peptide 可有效
的分辨登革病毒及日本腦炎病毒感染之老
鼠血清,這些抗原決定位的確認與研究,
將有助於epitope-based peptide antigen 之研
發,及了解出血性登革之病理機制。 | zh_TW |
dc.description.abstract | Serotype-specific monoclonal antibody
(MAb), D2 16-1 (Ab4), against dengue virus
type 2 (DEN-2) was generated. This MAb
recognized non-structural protein 1 (NS1) of
DEN-2; its specificity was determined by
ELISA, immunofluorescence and
immunoblotting analysis. Serotype-specific
B-cell epitope of Ab4 was further identified
from a random phage-displayed peptide
library. Selected phage clones specifically
reacted with Ab4 and did not react with other
MAbs. Immunopositive phage clones
displayed a consensus motif,
His-Arg/Lys-Leu/Ile. Synthetic peptide
corresponding to phage-borne peptide
specifically bound to antibody. His and Arg
in this epitope were crucial for peptide
binding to Ab4: binding activity dramatically
decreased when His or Arg was changed to
Leu. Epitope-based synthetic peptide
identified serum samples from
DEN-2-immunized mice and rabbits by
ELISA assay. The peptide clearly
differentiated between serum samples from
DEN-2- and Japanese encephalitis virus
(JEV)-immunized mice. This monoclonal
antibody and its epitope-based peptide
antigen will be useful for serologic diagnosis
of DEN-2 infection. Furthermore, DEN-2
epitope identification makes it feasible to
dissect the antibody response and to address
the role of antibodies in the pathogenesis of
primary and secondary DEN-2 infections. | en |
dc.format | application/pdf | zh_TW |
dc.format.extent | 99850 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language | zh-TW | zh_TW |
dc.language.iso | zh_TW | - |
dc.publisher | 臺北市:國立臺灣大學醫學院口腔生物科學研究所 | zh_TW |
dc.rights | 國立臺灣大學醫學院口腔生物科學研究所 | zh_TW |
dc.subject | 第二型登革病毒 | zh_TW |
dc.subject | 噬菌體顯現法 | zh_TW |
dc.subject | 抗原決定位 | zh_TW |
dc.subject | 登革偵檢試劑 | zh_TW |
dc.subject | monoclonal antibody | en |
dc.subject | B-cell
epitope | en |
dc.subject | synthetic peptide | en |
dc.subject | serologic
diagnosis | en |
dc.subject.classification | [SDGs]SDG3 | - |
dc.title | 研究第二型登革病毒血清型專一性抗體的B細胞抗原決定位及評估其為偵檢試劑之可行性 | zh_TW |
dc.type | journal article | en |
dc.identifier.uri.fulltext | http://ntur.lib.ntu.edu.tw/bitstream/246246/22667/1/912314B002257.pdf | - |
dc.coverage | 計畫年度:91;起迄日期:2002-08-01/2003-07-31 | zh_TW |
item.fulltext | with fulltext | - |
item.cerifentitytype | Publications | - |
item.openairetype | journal article | - |
item.languageiso639-1 | zh_TW | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.grantfulltext | open | - |
Appears in Collections: | 口腔生物科學研究所
|