https://scholars.lib.ntu.edu.tw/handle/123456789/162655
Title: | Age-Dependent Increase in C7-1 Gene Expression in Rat Frontal Cortex | Authors: | HUNG, HUI-CHUAN TSAI, MAY-J. WU, HAN-CHUNG LEE, EMINY-H.Y. |
Keywords: | C7-1;Regional distribution;Aging;V-ATPase;Frontal cortex | Issue Date: | 2000 | Journal Volume: | v.75 | Journal Issue: | n.2 | Start page/Pages: | 330-336 | Source: | MOLECULAR BRAIN RESEARCH | Abstract: | We have previously reported that a fragment of mRNA, denoted as C7-1, which expression was significantly increased in the frontal cortex of aged rats. In the present study, we have cloned and sequenced the full length cDNA of the C7-1 gene. We have found that the open reading frame of this gene encoded a 463-amino-acid protein, which shared 84% identity in amino acid sequence with a subunit of vacuolar H+-ATPase (V-ATPase). Further Northern blot analysis revealed that there was an age-dependent increase in C7-1 gene expression in rat frontal cortex, but not in other brain areas. Moreover, application of C7-1 antisense oligonucleotide to cortical neuronal cultures markedly inhibited cell survival. These results together suggest that C7-1 is a marker for the aging process and that upregulation of C7-1 may be important in maintaining the normal function of V-ATPase during aging.cortical neuronal cultures markedly inhibited cell survival. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/94753 |
Appears in Collections: | 口腔生物科學研究所 |
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