Viral Nucleic Acid Diagnostic Assays for Canine Infectious Respiratory Disease and Analysis of Clinical Cases
Date Issued
2006
Date
2006
Author(s)
Huang, Chiung-Yi
DOI
zh-TW
Abstract
Canine infectious respiratory disease (CIRD) is a general call of respiratory infections in dogs. Several studies of natural outbreaks of the disease have shown that the etiology is complex, with a variety of viruses and bacteria involved. The viruses that have been most frequently reported to be present are canine distemper virus (CDV), canine adenovirus type 1 and type 2 (CAV-1 and CAV-2), canine herpesvirus (CHV), canine parainfluenza virus type 2 (CPIV-2), and canine respiratory coronavirus (CRCoV). We detected these viruses from clinical samples of 54 cases with specific nucleic acid diagnostic assays based on RT-nPCR / n-PCR, and obtained the detection rates as follows: CDV: 41 %; CAV-2: 28 %; CPIV-2: 13 %; CRCoV: 8 %; CHV: 4 %. A population of dogs (26%) had evidence of infections with several viruses at the same time. The coinfection patterns are: CDV with CPIV-2 (9 %), CDV with CAV-2 (7 %), CAV-2 with CPIV-2 (4 %), and CDV with CHV (2 %). The infectious patterns involved with three viruses including: CAV-2, CHV and CRCoV (2 %); CDV, CAV-2 and CRCoV (2 %). Generally speaking, detection rates of nasal swab, oral swab and conjunctival swab have a elevation compared with whole blood. The gene sequence results of the RT-nPCR and n-PCR products of representative positive samples showed 98.7 – 100% nucleotide identity with corresponding viral gene and 95 – 100% with foreign strains. The nucleotides variation in CRCoV, CPIV-2, and CHV can be differentiated local strains form foreign strains. According to the statistical analysis results, any infectious pattern of these virus showed a significant variability with the severity of upper respiratory symptoms (P < 0.01), and higher variability in secondary bacterial infections (P < 0.01). In some particular viral infections showed a significant variability with the clinical sign of vomiting and diarrhea (P < 0.05)
Subjects
犬傳染性呼吸道疾病
canine infectious respiratory disease
SDGs
Type
thesis
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