https://scholars.lib.ntu.edu.tw/handle/123456789/163769
標題: | 行政院國家科學委員會補助專題研究計畫成果報告:鴨病毒性肝炎病毒之核酸定序與毒力因子分析 | 作者: | 蔡向榮 | 關鍵字: | Duck Viral Hepatitis;Genome;Picornavirudae;鴨病毒性肝炎:Duck Viral Hepatitis;基因體:Genome;小核醣核酸病毒:Picornavirudae | 公開日期: | 2002 | 出版社: | 臺北市:國立臺灣大學獸醫學系暨研究所 | 摘要: | In this project, complete genoine sequence of non type 1 duck viral hepatitis virus (DFIV) was resolved for the first time. It was showed that the genome of the DHV composed of 8, 283 bp, and the virus should be belonged to a new genus in Plcornaviriclae. The virus had a 6, 978 bp long open reading frame which was translation into a polyprotein with 2, 325 aa. The 3¡¦ NTR (non-translation-region) of the polyprotein was 235 bp in size and the poly A tail a size if 67 bp. It was showed that the amino acid sequence of DHV was most similar to enterovirus and rhinovirus in the Ficornaviridae family, but only with 25.5% and 24.3% homology. Besides, DHV possessed a 268 aa L protein which was lacking in both enterovirus and rhinovirus. Pylogenetic analysis of the amino acid sequence of the 3D and the polyprotein region, it was showed by a boostrap value of 99% and 100% that DHV should be belonged to a new genus in Ficornavirudae. 完成鴨病毒性肝炎(Duck Viral Hepatitis )病毒基因體8 , 283 bp 之核酸定序,由核酸定序之結果顯示此DVH 病毒應屬Picornavirudae之一員,但應是一新的病毒屬。DVH 病毒基因體是由一6 , 9 78 bp 之核酸序列組成之單一開放讀碼區,其轉譯成2 , 325 個氨基酸之polyprotein。於polyprotein 5端之5 ' NTR ( Non 一Translation 一Region )全長為l , 007 bp , 3 端之3 ' NTR 全長為235 bp ,至於poly -A tail 則為67 bp。 DVH 病毒之polyprotein 氨基酸序列經由比對之結果顯示,其與Picornavirudae 9個病毒屬中之enterovirus及rhinovirus較為相似,但一致性也分別只有25 . 5 %及24 . 3 %。而且於DVH 病毒之polyprotein 序列中具有一268 個氨基酸之L 蛋白質,此為enterovirus 及rhinovirus 所未具備的。此外以DHV 病毒之3D 及polyprotein之氨基酸序列行演化樹之分析,結果發現DVH 病毒均應為一獨立之病毒屬,而且其bootstrap 值均高達99 %與1 00 % ,因此我們相信DVH 病毒應為Picornavirudae的一個新的病毒屬。 |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/28708 | 其他識別: | 902313B002312 | Rights: | 國立臺灣大學獸醫學系暨研究所 |
顯示於: | 獸醫學系 |
檔案 | 描述 | 大小 | 格式 | |
---|---|---|---|---|
902313B002312.pdf | 696 kB | Adobe PDF | 檢視/開啟 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。