https://scholars.lib.ntu.edu.tw/handle/123456789/163772
Title: | 行政院國家科學委員會補助專題研究計畫成果報告:抗氧化物 Silymarin 對免疫細胞的作用 | Authors: | 林大盛 | Keywords: | silymarin;免疫調控;細胞免疫;Th1/Th2 型細胞素;immunomodulation;cell-mediated immunity;Th1-/Th2-type cytokines | Issue Date: | 2002 | Publisher: | 臺北市:國立臺灣大學獸醫學系暨研究所 | Abstract: | Milk thistle (Silybum marianum) 有效 的萃取物為silymarin,乃民眾最常用來治 療肝病之草藥。一些證據指出silymarin 作 用類似維生素E,亦為membrane-active antioxidant,可保護細胞膜因H2O2 及其他 自由基的傷害,亦有提升淋巴細胞及紅血 球之superoxide dismutase 活性,因而認為 silymarin 之抗氧化作用為其肝臟保護能力 之一重要因子。另外,一些研究也指出 silymarin 可影響淋巴細胞的活化,增強 lectin 對淋巴細胞的作用,提示了silymarin 亦可藉由影響體內免疫細胞反應,進而達 到肝臟保護效果。雖然silymarin 對肝臟之 療效尚待進一步臨床實驗證實,但如上所 述,在世界各地silymarin 已普遍被用於治 療人及動物肝相關的疾病,故有必要了解 其對體內免疫系統之影響。由於silymarin 各種活性類似維生素E,而維生素E 已被 證實並非只是單純的抗氧化物,亦為免疫 調控物質, 故本研究之目的在探討 silymarin 是否亦為免疫調控物質。即探討 silymarin 影響巨噬細胞分泌IL-1 及 tumorcidal activity , 刺激lymphocyte proliferation,以及影響Th1/Th2 型免疫反 應。 結果發現Silymarin 本身對淋巴細胞有 直接刺激效果,在同時有PHA 存在下刺激 效果會更好。同時,silymarin 有助於受刺 激細胞Th1 及Th2 型之反應,但前者效果 比後者好。Silymarin 在LPS 刺激下可增強 巨噬細胞活性。 Silymarin, the active extract of milk thistle (Silybum marianum), has been used for the treatment of liver diseases for centuries. It is evident that biological activities of silymarin are similar to vitamin E being membrane-active antioxidant. Silymarin can protect cell membrane damage done by H2O2 and other free radicals and also can enhance the superoxide dismutase activity of lymphocytes and erythrocytes. Therefore, it is believed that antioxidant activity is an important ability of silymarin for liver protection. In addition, some studies indicate that silymarin can influence lymphocyte activation and enhance lectin-induced lymphoblast transformations. Thus, it is suggested that silymarin may also offer liver protection ability by influencing immune cell responses. Although the efficiency of silymarin for the therapy of liver diseases still needs verification by further human clinical trials, as described above silymarin has already been used widely for the therapy of human and animal liver diseases. Therefore, it is necessary to understand the effects of silymain on immune system in the body. Because biological activities of silymarin are similar to vitamin E, which has been proved to be an immunomodulator, the purpose of this study will investigate if silymarin also an immunomodulator as well. The following items will be examined for the effects of silymarin on immunity: IL-1 secretion and tumorcidal activity of macrophages, stimulation of lymphocyte proliferation, and influence of Th1/Th2 type immune responses. It was found that silymarin could directly stimulate lymphocyte proliferation. This effect would be significantly enhanced when PHA was also present. At the same time, silymarin promoted both Th1 and Th2 responses. However, the former was enhanced greater than the latter. Silymarin plus LPS could enhance both IL-1 secretion and tumorcidal activity of macrophages. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/28711 | Other Identifiers: | 902320B002118 | Rights: | 國立臺灣大學獸醫學系暨研究所 |
Appears in Collections: | 獸醫學系 |
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902320B002118.pdf | 149.52 kB | Adobe PDF | View/Open |
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