Pathology and Genotyping Characterization of Bovine Tuberculosis in Taiwan
Date Issued
2009
Date
2009
Author(s)
Fu, Dah-Jiun
Abstract
The campaign against bovine tuberculosis has been carried out in Taiwan based on a principle of test-and-slaughter program for more than 50 years, however, sporadic outbreaks contributed to a fluctuant prevalence of tuberculin test reactors still remained on the present day. A total of 57 cattle with skin test-positive reaction, which originated from 4 individual farms in a central-western county in Taiwan, were slaughtered and sampled for pathologic investigation, molecular diagnosis and bacterial strains genotyping. Tuberculous lesions were identified in 27 cattle (47.4%; n = 57) with the evidence on gross and/or microscopic examination in 1 or more sites. The most frequently affected site was retropharyngeal lymph node, followed by mediastinal lymph node, hilar lymph node, and mesenteric lymph node. Most of the etiologies of these tuberculous lesioned cattle (88.9%; 24/27) were identified as Mycobacterium bovis infection through the PCR assays. Three genotypes of Mycobacterium bovis were found among fifteen skin test positive cattle from three different farms by a molecular genotyping method based on variable number tandem repeat (VNTR) analysis. The findings uncovered the infective status of bovine tuberculosis in the cattle under routine tuberculin test survey, and suggested the application of VNTR to use in distinguish variant strains of Mycobacterium bovis in Taiwan.
Subjects
VNTR genotyping
molecular diagnostic
pathologic investigation
Taiwan
tuberculosis
SDGs
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-98-R96629011-1.pdf
Size
23.32 KB
Format
Adobe PDF
Checksum
(MD5):d240ec56a506302c9a43c43aaf0bdb51