https://scholars.lib.ntu.edu.tw/handle/123456789/164654
標題: | PCR-based restriction fragment length polymorphism (RFLP) analysis of Campylobacter jejuni isolates from humans, chickens and dogs in northern Taiwan. | 作者: | Tsai H. J.*, H. C. Huang, H. L. Tsai, C. C. Chang Tsai, H.-L. HSIANG-JUNG TSAI |
公開日期: | 一月-2006 | 卷: | 68 | 期: | 8 | 起(迄)頁: | 815-819 | 來源出版物: | J. Vet. Med. Sci | 摘要: | Two hundred and twenty strains of Campylobacter jejuni (70 human, 51 canine and 99 chicken strains) were isolated from September 2003 to September 2004 in northern Taiwan. These strains were subtyped by PCR-RFLP analysis of the flagellin (FlaA) gene. On the basis of restrictive digest, six types were identified with AfaI, seven types with MboI and five types with HaeIII. With the combination of these three enzymes, 47 distinct PCR-RFLP patterns were observed - 25 each from human and chicken isolates, and 9 from canine isolates. In human strains, the most frequently occurring types were Cj-28 (14.3%), Cj-17 (10%), Cj-16 (8.6%), Cj-37 (7.1%) and Cj-46 (7.1%). In canine strains, the most prevalent types were Cj-1 (33.3%), Cj-26 (19.6%), Cj-3 (15.7%), Cj-2 (9.8%) and Cj-10 (9.8%). In chicken strains, the most frequently occurring types were Cj-46 (40.4%), Cj-29 (9.1%), Cj-45 (7.1%) and Cj-41 (5.1%). The results suggest that poultry is a source, but not the sole source, of C. jejuni infection in humans. Two RFLP types, Cj-17 and Cj-37, frequently occurring in human isolates in this study have also been found to be prevalent in human isolates in Japan, China and the Czech Republic, indicating a possible international clonal spread. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/199596 | DOI: | 10.1292/jvms.68.815 | SDG/關鍵字: | animal; animal disease; article; bird disease; Campylobacter jejuni; chicken; classification; dog; dog disease; genetics; Gram negative infection; human; isolation and purification; microbiology; restriction fragment length polymorphism; Taiwan; Animals; Campylobacter Infections; Campylobacter jejuni; Chickens; Dog Diseases; Dogs; Humans; Polymorphism, Restriction Fragment Length; Poultry Diseases; Taiwan; Campylobacter jejuni; Canis familiaris; Gallus gallus |
顯示於: | 獸醫學系 |
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